摘要
目的 探讨流式细胞术 (FCM)三色分析血液中循环活化血小板 (CAP)在缺血性脑血管病中的临床意义。方法 以纤维蛋白原受体 (FIB R)、P 选择素 (CD6 2P)作为分子标志物 ,FCM三色免疫荧光分析CAP。结果 正常人静止血小板膜表面FIB R和CD6 2P表达极低 (阳性百分率的中位数分别为 1.4%和 0 .7% )。 0 .1μmol/LADP可使血小板明显活化 ,10 μmol/LADP使活化血小板膜表面FIB R和CD6 2P表达至峰值 ,阳性百分率分别为 87.8%和 81.3%。合成肽 (RGDS)可抑制抗FIB R的单克隆抗体与ADP活化血小板结合。 112例有血栓形成倾向疾病和 12 0例缺血性脑血管病患者血液中表达FIB R的CAP数量与正常人比较差异有显著意义 (P <0 .0 1) ,但表达CD6 2P的CAP数量增高不明显 (P >0 .0 5 )。急性脑梗死患者治疗前后CAP的FIB R表达呈连续下降趋势 (P <0 .0 5 ) ,而且CAP的FIB R表达量与患者的ESS(欧洲脑卒中评分 )呈负相关。结论 FIB R可作为CAP检测的灵敏、特异的分子标志物 ,流式细胞术三色分析CAP能准确地反映体内血小板的活化水平 ,对缺血性脑血管病的发病机理研究、预后判断。
Objective To explore the flow cytometric 3 color analysis of circulating activated platelets (CAP) and its clinical significance in ischemic cerebrovascular diseases. Methods The fibrinogen receptor (FIB R) and P selectin (CD62P) were used for molecular marker of CAP, which were analyzed by flow cytometric 3 color immunofluorescence. Results Expression of FIB R and CD62P on the resting platelet membrane surface was very lower in normal individuals, the median of FIB R and CD62P positive percent was 1.40% and 0.70% respectively. Platelet can be obviously activated by 0.1 μmol/L ADP, and 10.0 μmol/L ADP leaded to the peak expression of FIB R and CD62P on the activated platelet membrane surface, FIB R and CD62P positive percent of 87.8% and 81.34% respectively. That monoclonal antibody of the FIB R combined with ADP activating platelets was inhibited by synthetic peptide (RGDS). The quantity of CAP with FIB R expression was markedly higher ( P <0.01) and the amount of CAP with CD62P expression was not significantly higher ( P >0 05) in blood of 112 patients with thrombotic tendency and 120 patients with ischemic cerebrovascular diseases than in healthy individuals. The expression of CAP with FIB R of in front and behind treatment of acute cerebral infarction continuously decreased ( P <0.05), and the quantity of CAP with FIB R expression was related negatively to Europe stroke score (ESS) of patients with acute cerebral infarction. Conclusion The FIB R may be regarded as sensitive and specific molecular marker in detection of CAP. The level of platelet activation in vivo can be reflected accurately by flow cytometric 3 color analysis of CAP, which has an important clinical significance for the study of pathogenesis, estimation of prognosis and supervision of curative effect in ischemic cerebrovascular diseases.
出处
《中华医学杂志》
CSCD
北大核心
2000年第7期499-502,共4页
National Medical Journal of China
基金
国家卫生部科学研究基金资助项目!(961261)
关键词
流式细胞术
血小板活化
FIB-R
缺血性脑血管病
Flow cytometry
Activated platelet
Fibrinogen receptor
P-selectin
Ischemic cerebrovascular diseases
