摘要
提要从东海陆架DH-13站点的表层沉积物中提取环境基因组DNA,通过PCR和TA克隆构建了细菌和古菌的16SrDNA基因文库,并对克隆子文库进行系统发育分析。结果表明:细菌序列以变形菌门(Proteobacteria,41.5%)居多,其次是浮霉菌门(Planctomycetes,10.9%)、放线菌门(Actino-bacteria,8.9%)和CFBgroup(7.9%),另外还有少量酸杆菌门(Acidobacteria)、疣微菌门(Verru-comicrobia)、硝化螺旋菌门(Nitrospira)和厚壁菌门(Firmicutes)等;古菌序列全部来自泉古生菌门(Crenarchaeota),其中Marine Crenarchaeotic Group Ⅰ(MGⅠ,93.6%)是绝对优势菌,还有少量序列属于Miscellaneous Crenarchaeotic Group(MCG)、Marine BenthicG roup C(MBGC)和Marine Benthic Group A(MBGA)。文库多样性分析结果显示东海陆架表层沉积物中有着丰富多样的微生物群落,细菌的多样性更为显著。
The environmental total DNAs were directly extracted from the surface layer sediment of the East China sea. The bacterial and archaeal 16S rDNA gene libraries were generated by using PCR and TA cloning. Phylogenetic results show that there are eight phyla in the bacteria domain： Proteobacteria （41.5%）, Planctomycete （10.9%）, Actinobacteria （8.9%）, CFB group （7.9%）, Acidobacteria, Verrucomicrobia, Nitrospira and Firmicutes, the phylum Proteobacteria is pre- dominant. In the archaea domain, all clones were belonged to Crenarchaeota and no one Euryarchaeota clone was detected. There are four divisions in the Crenarchaeota kingdom： Marine Crenarchaeotic Group I （MG I, 93.6%）, Miscellaneous Crenarchaeotic Group （MCG）, Marine Benthic Group C （MBGC）, Marine Benthic Group A （MBGA）, and MG I is the dominating group. DOTUR statistics of 16S rDNA gene sequences from the libraries indicating a high richness in the sediment microbial communities.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2012年第4期805-813,共9页
Oceanologia Et Limnologia Sinica
基金
浙江省自然科学基金项目
LY12C03003号
浙江省科技厅公益性技术应用研究项目
2011C31017号
舟山市科技局海洋类项目
10248号
浙江省大学生科技创新项目
2010R411002号
