摘要
目的:建立高效液相色谱法测定赤牡泡腾片中芍药苷含量的方法。方法:采用Zobax Eclipse XDB-C_(18)(250 mm×4.6 mm,5μm)色谱柱,乙腈-水(11:89)为流动相,流速:1.0 ml·min^(-1),柱温:30℃,检测波长230 nm。结果:芍药苷的线性范围为5.06～80.96μg·ml^(-1)(r=0.999 9)。平均回收率为98.30%(RSD=0.91%,n=6)。结论:本方法准确、简便、快速,能用于测定赤牡泡腾片中芍药苷的含量。
Objective： To establish an HPLC method for the determination of paeoniflorin in Chimu effervescent tablets. Method ： The determination was performed on a Zobax Eclipse XDB-C18 （250 mm ×4.6 mm,5μm ） column, acetonitrile-water （11：89） was the mobile phase with the flow rate of 1.0 ml ·min-1 and the column temperature of 30℃ , and the detection wavelength was at 230 nm. Result ： The linear range of paeoniflorin was within the range of 5.06 - 80.96 μg · ml - 1 （ r = 0. 9999 ）. The average recovery was 98.30% （RSD =0.91% , n =6）. Conclusion： The method is accurate, simple and rapid, and can be used for the determination of paeoniflorin in Chimu effervescent tablets.
出处
《中国药师》
CAS
2012年第7期978-979,共2页
China Pharmacist
