摘要
目的:对分别采自云南、新疆两个地区的大麻植物,通过ITS序列的分析,从分子水平进行鉴定。方法:采用DNA提取试剂盒提取大麻的ITS全序列DNA,经过PCR扩增,胶回收后测序,所得序列与从Genbank中获取3个大麻科植物的ITS全序列比对,以葎草的ITS全序列为外源基因构建了大麻属的系统发育树。结果:对获得的基因序列进行同源性分析,NCBI数据库中登陆的大麻相应序列与新疆大麻(毒品大麻)的ITS全序列亲缘关系性最近,为99%;云南大麻(工业大麻)与新疆大麻(毒品大麻)亲缘关系较远。结论:ITS序列能够较准确的将毒品大麻和工业大麻区分开。
objective：To identify cannabis sativa plants from Yunnan and Xinjiang areas on molecular level by internal transcribed spaces（ITS） sequence analysis.Methods：The complete ITS sequencing of cannabis sativa was determined through DNA extraction by Plant Genomic DNA Kit,PCR amplification,glue recovery and sequencing.Then the phylogenetic tree of cannabinaceae was constructed by comparing with the complete ITS sequencing of three cannabis plants from GenBank.Results： The gene sequence of cannabis by homology analysis showed that the genetic relationship between NCBI database and Xinjiang cannabis（drugs hemp） was the nearest,the homology reaches 99%;but the relationship between Yunnan cannabis（industrial hemp） and Xinjiang cannabis is farther.Conclusion： The cannabis from different sources could be identified by ITS sequence.
出处
《泸州医学院学报》
2012年第4期364-367,共4页
Journal of Luzhou Medical College
基金
四川省教育厅科研项目(09ZC053)
泸州市科技局重点项目(2010-S-35)
关键词
毒品大麻
工业大麻
ITS全序列
系统发育树
Marijuana hemp
Industrial hemp
Internal transcribed spaces
Phylogenetic tree
