摘要
为了研究比较接种和不接种丛枝菌根(AM)真菌对滇重楼根茎中甾体皂苷类成分的影响,通过室温盆栽接种试验,采用高效液相色谱法测定并比较收获滇重楼根茎中甾体皂苷类成分的差异。结果显示,不同AM真菌对滇重楼根茎中次生代谢产物甾体皂苷的影响存在差异。AM真菌诱导子处理后,没有改变滇重楼根茎的化学背景,但能部分提高其根茎中化学成分的含量。实验结果表明,AM真菌与滇重楼之间具有选择性,根内球囊霉是最适宜滇重楼接种的优良菌种。
To compare the effects of inoculated or non-inoculated with arbuscular mycorrhizal （AM） fungi on the steroidal saponin component in root of Paris polyphylla vat. yunnanensis. By pot experiments, steroid saponin component in root of P polyphylla var. yunnanensis was determined and compared by HPLC. The results showed there was difference in the effects of different AM fungal on the secondary metabolite steroid saponin in P. polyphylla var. yunnanensis. After elicitors treatment, AM fungal did not change the chemical backgrounds of P. polyphylla vat. yunnanensis, but can improve partly the content of chemical compositions in roots. In conclusion, there was selectivity between AM fungal and P polyphylla vat. yunnanensis. Glornus intraradices was the most appropriate strain for inoculation P. polyphylla var. yunnanensis.
出处
《药学学报》
CAS
CSCD
北大核心
2012年第9期1237-1242,共6页
Acta Pharmaceutica Sinica
基金
云南省科技厅科研项目(2011FB081)
重庆市高校创新团队建设计划项目(201040)
四川省教育厅科研项目(09ZA176)
四川省中医药管理局科研项目(2010-89)
关键词
滇重楼
AM真菌
甾体皂苷
次生代谢
指纹图谱
Paris polyphylla vat. yunnanensis
arbuscular mycorrhizal fungi
steroidal saponin
secondary metabolites
fingerprint
