摘要
以小鼠垂体蛋白质为材料 ,用固相 p H梯度等电聚焦和 SDS- PAGE获得蛋白质组双向凝胶电泳图谱 .以已知等电点和相对分子质量的外标和内标做为参照物 ,经过 PDQUEST软件对凝胶图像进行计算机分析 ,获得了小鼠垂体蛋白质组的等电点、相对分子质量和相对含量等参数 .
Employing isoelectric focusing on immobilized pH gradients followed by SDS PAGE,a silver stained 2D gel of mouse hypophysis was obtained. The image of the 2D gel was processed by computer. The protein spots were detected and quantificated by the PDQUEST software from Bio rad Inc. The pI and M r of the proteins on the gel were calibrated according to the pI and M r of the inner standard proteins. The results provide a basis for the further identification of the protein spots on the 2D gel of the mouse hypophysis.
出处
《生命科学研究》
CAS
CSCD
2000年第2期118-123,共6页
Life Science Research
基金
国家自然科学基金资助项目!(39990 60 0 )
