摘要
为研究ｇｒｐ７５的功能，对过表达ｇｒｐ７５的ＣＨＬ细胞进行无糖培养以施加能量代谢应激，运用台盼蓝染色计数、ＬＤＨ释放测定和流式细胞术等方法评估其损伤程度。结果显示，无糖培养５ｈ，过表达ｇｒｐ７５细胞和对照组细胞比较，细胞活率、亚二倍体细胞率均无明显差别；无糖培养 １０ｈ，过表达ｇｒｐ７５ 细胞的活率高于对照组（Ｐ＜ ０．０１），亚二倍体细胞率低于对照组（Ｐ＜０．０５）；无糖培养至２０ｈ，两组细胞活率和亚二倍体细胞比率无显著差别。ＬＤＨ释放测定结果显示，无糖培养４ｈ，过表达ｇｒｐ７５细胞和对照组细胞ＬＤＨ释放百分比无显著差别；无糖培养７～１５ｈ，过表达ｇｒｐ７５细胞ＬＤＨ释放百分比低于对照组（Ｐ＜０．０５）；无糖培养２０ｈ，两组细胞ＬＤＨ释放百分比无显著差别。这一研究结果表明，过表达ｇｒｐ７５的细胞对一定强度的缺糖损害有明显的耐受性（表现为较轻的细胞膜损伤，较轻的ＤＮＡ断裂和较高的存活率），即细胞内ｇｒｐ７５具有对抗缺糖损伤的作用。
To elucidate the functions of grp75, CHL cells over-expressing grp75 are cullled in glucose-free medium in order to simulate energy metabolic stress. Their susceptibilities to injuries caused by glucose deprivation are assessed by trypan blue exclusion, LDH leakage measurement and cytometry analysis. Data shows a stronger resistance to glucose deprivation among cells over-expressing grp75 than among cells constitutively expressing grp75. The outcome suggests that grp75 can protect cells from injuries caused by glucose deprivation.
基金
国家自然科学基金!(39670239)
美国中华医学基金!(96-635)
