摘要
以复叶槭的茎段、叶片为外植体,以MS为基本培养基,附加多种激素配比试验,结果表明:叶外植体在各种培养基上均形成愈伤组织,但没有出现芽的分化。茎段外植体在多种培养基上均产生愈伤组织,但仅在MS+0.000 5mg.L-1TDZ+0.01 mg.L-1NAA培养基上出现再生芽,芽的分化率为22.8%,在MS+0.1 mg.L-1NAA上生根率为83.3%,移植后成活率为86.2%。
The stem segments and leaves of Acer negundo as explants were put on MS basis culture media with various hormones matching. The results indicated that the callus was induced from leaves on all kinds of culture media, but without the adventitious buds obtained. The callus induced from stem segments was found on several types of culture media, while the regeneration buds were only found on MS ＋0.000 5 mg·L^-1 TDZ ＋ 0.01 mg·L^-1 NAA and differentiation ratio of buds was 22.8%. The rooting rate was 83.3% on MS ＋ 0.1mg·L^-1NAA. The survival rate of transplantion was 86.2%.
出处
《林业科学研究》
CSCD
北大核心
2012年第4期516-520,共5页
Forest Research
基金
中央高校基本科研业务费专项资金项目(DL12CA37)
