摘要
目的回顾性分析慢性HBV感染者肝细胞内HBV ccc DNA含量与肝细胞tDNA、HBsAg、HBcAg的关系以及临床意义。方法应用荧光定量聚合酶链反应(FQ-PCR)检测未经抗病毒治疗的患者58例(未抗病毒组)、经抗病毒治疗的慢性乙型肝炎患者4例(抗病毒组)的肝细胞HBV ccc DNA和tDNA含量,应用免疫组化法定性检测肝组织内HBsAg和HBcAg表达,用SPSS13.0软件统计分析肝细胞HBV ccc DNA含量、tDNA、HBsAg、HBcAg间的关系。结果肝细胞HBV ccc DNA、HBV tDNA含量与HBsAg、HBcAg在肝细胞中的表达模式间差异无统计学意义(P>0.05)。肝细胞内HBV ccc DNA含量与HBV tDNA含量呈高度正相关(P<0.01)。慢性乙型肝炎抗病毒治疗患者中,疗程>2年并达到《病毒性肝炎防治方案》停药标准,肝细胞内ccc DNA、tDNA含量较未抗病毒组显著降低,但肝细胞内仍然可以检出HBV ccc DNA、tDNA。结论肝细胞HBV ccc DNA含量与HBsAg、HBcAg在肝细胞的表达模式间无一定规律性。核苷(酸)类似物抗病毒治疗2～3年并不能完全清除肝细胞内HBV ccc DNA。
Objective The correlation between the quantity of HBV ccc DNA and tDNA, HBsAg, HBcAg in the hepatocytes of chronic hepatitis B patients and the clinical significance were analyzed, retrospectively. Methods Patients in this study were divided into two groups： nonantiviral therapy group （58 cases ）, antiviral group （4 patients with chronic hepatitis B after nucleos （t）ide therapy）. The quantity of HBV ccc DNA and tDNA in the hepatocytes of the two groups were detected by FQ-PCR, the levels of HBsAg, HBeAg in the hepatoeytes were identified by immunohistoehemistry. The correlation between quantity of HBV tee DNA and tDNA, HBsAg, HBcAg in the hepatocytes of chronic hepatitis B patients were analyzed by SPSS 13.0 software. Results There were no significant difference between the quantity of HBV eee DNA, tDNA in the hepatocytes and the expressive mode of HBsAg, HBcAg （P 〉 0.05 ）. There was a significant positive correlation between the quantity of HBV ecc DNA and HBV tDNA in the hepatocytes （P 〈 0.01 ）. Patients accepted nuclesos （t）ide analogue therapy over 2 years and reached the criteria of therapy endpoint in China guideline for hepatitis B patients. The quantity of HBV ccc DNA and tDNA in the hepatocytes were lower than that of the patients without antiviral therapy, but the HBV ccc DNA and tDNA in the hepatocytes could be detected yet. Conclusions There were no regularity between the quantity of HBV ccc DNA, tDNA in the hepatoeytes and the expressive mode of HBsAg, HBeAg. HBV ecc DNA in the hepatocytes could not be cleared completely through nucleoside analogue therapy for 2-3 years.
出处
《中华实验和临床感染病杂志（电子版）》
CAS
2012年第4期34-37,共4页
Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)
基金
广西自然科学基金(No.2010GXNSFD013046
No.桂科自0832117
No.桂科自0542092
No.桂科青0832037)
广西医学科学实验中心开放基金资助项目(No.KFJJ2010-20)
