摘要
目的:观察不同浓度含己烯雌酚(DES)血清对体外培养人黑素细胞的增殖和酪氨酸酶活性的影响。方法:采用MTT法测定黑素细胞增殖情况;参考Nakajima M方法测定酪氨酸酶活性;NaOH溶解法测定黑素含量。结果:己烯雌酚血清不同剂量对人黑素细胞增殖及酪氨酸酶活性均有促进作用。结论:己烯雌酚能促进体外培养人黑素细胞增殖及酪氨酸酶活性,对相关皮肤色素性疾病可能有一定的治疗作用。
Objective Establishing the culture of human normal melanocytes in vitro,and studying the effects of Serum containing Diethylstilbestrol（DES）on cultured human melanocytes.Methods Viability of melanocytes were measured by MTT method;tyrosinase activity was measured by utilization of L-Dopa as the substrate（Nakajima M method） and melanocytes melanogenesi by NaOH method.Results Different dosage of Serum containing Diethylstilbestrol（DES）induced a strong promotion on melanocytic viability and tyrosinase activity.Conclusion Serum containing Diethylstilbestrol（DES）can promote melanocytic proliferation and tyrosinase activity.Serum containing Diethylstilbestrol might have some therapentical effects on correlated pigmented skin diseases.
出处
《中国美容医学》
CAS
2012年第9期1527-1529,共3页
Chinese Journal of Aesthetic Medicine
基金
海南省卫生厅科学研究课题(琼卫2010-23)
