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吴茱萸中吴茱萸次碱的提取工艺及含量测定 被引量:3

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摘要 目的:建立用高效液相色谱法(HPLC)测量中药吴茱萸中吴茱萸次碱含量的方法。方法:以甲醇为溶剂,进行不同提取方法对吴茱萸次碱提取试验,设计正交试验,考察浸泡时间、提取时间、溶剂用量为3因数,采用L9(34)正交试验表,HPLC法检测。色谱柱:Welchrom-C18(5μm,250 mm×4.6 mm);流动相:乙腈—水(67:33),流速:1.0 ml/min,柱温:22℃,检测波长:290 nm。结果:回流提取法提取效率较高,吴茱萸次碱的最佳提取条件为用100 ml甲醇浸泡30 min回流提取75 min。吴茱萸次碱的回归方程为Y=2×106X-9078.7,相关系数r=0.9999,其线性范围为0.1002~1.1952μg,平均回收率为100.21%,RSD1.47%。吴茱萸中吴茱萸次碱的含量为0.279%。结论:方法稳定、重现性好、操作简单、结果准确,是检测吴茱萸中吴茱萸次碱较理想的方法。
出处 《微量元素与健康研究》 CAS 2012年第5期14-16,共3页 Studies of Trace Elements and Health
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共引文献26

同被引文献43

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