摘要
目的:建立同时测定人参中17种游离氨基酸和总氨基酸含量的方法,并应用于不同生长周期、炮制方法和生长部位的人参研究。方法:采用异硫氰酸苯酯对样品进行柱前衍生,以反相高效液相色谱法测定,外标法计算含量。色谱柱为UltimateAAA氨基酸分析专用柱(250mm×4.6mm,5μm),流动相A为三水合醋酸钠缓冲溶液(pH6.5)-乙腈溶液(93:7,V/V)、B为80%乙腈-水(80:20,V/V),梯度洗脱,检测波长为254nm。结果:17种氨基酸的检测浓度线性范围为0.0025～2.5000μmoL·mL-1,r均>0.9980。17种游离氨基酸的平均加样回收率在87.30%～100.01%之间,RSD在0.59%～2.94%之间(n均为6);测定总氨基酸时的平均加样回收率在91.50%～100.07%之间,RSD在0.41%～2.00%之间(n均为6)。结论:本方法灵敏、准确、重复性好,可用于人参及其相关产品的质量控制。
OBJECTIVE： To develop the method for the content determination of 17 kinds of free amino acid and total amino acid in Panax ginseng, and to apply the method in the study of P. ginseng in different growth period and parts processed by meth- ods. METHODS： The samples were derived with phenyl isothiocyanate. HPLC method was adopted and external method was used for calculating the content of sample. Ultimate AAA column（250 mm×4.6 mm, 5 μm） were adopted with mobile phase A consisted of sodium acetate trihydrate solution （pH 6.5）-acetonitrile（93 ： 7, V/V） and mobile phase B consisted of 80% acetonitrile-water（80 ： 20, V/V） （gradient elution）. UV wavelength was set at 254 urn. RESULTS： The linear range of 17 kinds of amino-water was 0.002 5-2.500 0 μmoL.mL^-1（r〉0.998 0） with an average recovery of 87.30%-100.01% （RSD=0.59%-2.94% ,n=6）; the average recovery of total amino acids was 91.50%-100.07% （RSD=0.41%-2.00% ,n=6）. CONCLUSION： The method is sensi- tive, accurate and reproducible, and can be used for quality control of P. ginseng and its products.
出处
《中国药房》
CAS
CSCD
2012年第35期3334-3337,共4页
China Pharmacy
