摘要
A maize (Zea mays L.) genome_specific repeated DNA sequence (clone MR64) has been transferred into one DH line of wheat through wheat (Triticum persicum Vav. ex Zhuk.) and maize cross. In the present study by RFLP analysis the authors proved that this DNA sequence could stably transmit into DH3 plants, the next generation derived from DH2 self_crossing. A similarity search in all DNA databases using BLASTN program showed that the DNA sequence of MR64 had as high as 93% identity to PREM_2 and 79% to Opie_2 in nucleotides. Both PREM_2 and Opie_2 are known as retrotransposons in maize genome, suggesting that MR64 likely is the partial sequence of a maize retrotransposon. Therefore, the results indicate that some retrotransposon might involve the DNA introgression from maize to wheat genome through wide fertilization. Stable inheritance of this maize genome_specific retrotransposon_like DNA in the wheat genome opens up the possibility of using retrotransposon as a new tool for gene tagging, function analysis, and insertional mutagenesis in wheat genome.
作者曾报道了一个玉米 (ZeamaysL .)基因组特异的重复序列DNA(克隆MR6 4)通过小麦 (TriticumpersicumVav .exZhuk .)与玉米杂交导入一个小麦加倍单倍体 (DH)植株中。利用RFLP分析证实该重复DNA序列可以稳定地传递到小麦DH3代植株。通过Internet在DNA数据库中进行序列相似性搜寻和同源性比较 ,结果显示 ,MR6 4的DNA序列和玉米的最近报道的两个逆转座子PREM_2和Opie_2分别有高达 93%和 79%的序列同一性。因此 ,该玉米DNA片段很可能是一种逆转座子的一部分 ,这意味着有可能利用玉米的某些基因组特异的逆转座子的序列在小麦基因组中进行基因标签、功能分析和插入突变的研究。
