摘要
目的:对纳豆菌抗菌脂肽抑制副溶血弧菌的抑菌机理进行探讨。方法:通过纳豆菌抗菌脂肽对副溶血弧菌生长曲线、细胞超微结构、细胞膜通透性、细胞DNA的作用、细胞蛋白的合成、细胞代谢的影响等方面的研究,探讨纳豆菌抗菌脂肽抑制副溶血弧菌的机理。结果:抗菌脂肽可以使副溶血弧菌细胞膜通透性增加,使得细胞内具有紫外吸收的大分子物质泄漏到细胞外;扫描电镜结果表明抗菌脂肽可以导致副溶血弧菌细胞壁破裂或形成孔洞;抗菌脂肽能够和副溶血弧菌DNA体外结合,导致DNA最大吸收峰发生了轻微的蓝移,且产生增色效应;SDS-PAGE分析表明抗菌脂肽对副溶血弧菌蛋白表达未见有明显影响;当抗菌脂肽的质量浓度达到0.6 MIC以上时,其对副溶血弧菌代谢活力的抑制作用显著。结论:纳豆菌抗菌脂肽可使副溶血弧菌的细胞膜通透性增加,或形成孔洞导致细胞内一些物质泄漏,使细胞生长受到抑制,进而导致死亡。
Objective：To investigate the antibacterial mechanism of lipopeptide from Bacillus natto against Vibrio parahemolyticus.Method： The effects of antibacterial lipopeptide from Bacillus natto on the growth,cell ultrastructure,cell membrane permeability,protein synthesis,and respiratory metabolism of Vibrio parahemolyticus were evaluated.Results： The antibacterial lipopeptide increased cell membrane permeability of Vibrio parahemolyticus,causing intracellular macromolecules with ultraviolet absorption to leak out of the cells.Under scanning electron microscope,it was observed that the cell wall of Vibrio parahemolyticus was ruptured by the antibacterial lipopeptide and holes were formed.The antibacterial lipopeptide could be bound to DNA from Vibrio parahemolyticus in vitro,resulting in slight blue shift of DNA maximum absorption and hyperchromic effect.SDS-PAGE suggested that the antibacterial lipopeptide had no apparent effect on protein synthesis in Vibrio parahemolyticus cells.The respiratory metabolism of Vibrio parahemolyticus was strongly inhibited by the antibacterial lipopeptide when its concentration was higher than 0.6 MIC.Conclusion： The antibacterial lipopeptide from Bacillus natto increases cell membrane permeability of Vibrio parahemolyticus,causing intracellular materials to leak out of the cells and consequently resulting in growth inhibition and even death of the cell.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第15期201-205,共5页
Food Science
基金
国家自然科学基金面上项目(30972287)
关键词
抗菌脂肽
纳豆菌
副溶血弧菌
抑菌机理
antibacterial lipopeptide
Bacillus natto
Vibrio parahemolyticus
antibacterial mechanism
