摘要
目的:建立不同产地金盏银盘药材的高效液相色谱指纹图谱,并同时测定金丝桃苷、异槲皮苷、鬼针聚炔苷和紫云英苷4种主要成分的含量。方法:采用反相高效液相色谱法,选用Hypersil ODS C18(250 mm×4.6 mm,5μm)色谱柱,乙腈-3%冰醋酸为流动相梯度洗脱,采用药典委员会颁布的中药色谱指纹图谱相似度评价系统(2004 A)评价图谱的相似性,同时测定药材中4种主要成分的含量。结果:建立了不同产地金盏银盘药材的HPLC指纹图谱,方法学符合指纹图谱要求,不同产地药材相似度差异不大,但4种主要成分含量差异较大。结论:该方法稳定、准确、可靠,指纹图谱结合含量测定为金盏银盘药材质控标准的制定提供了科学依据。
Objective：To establish HPLC fingerprint of Bidens biternata from different habitats and determine the contents of hy- peroside, isoquercetin, astragalin and bipinnatapolyacetylpside. Methods： Analysis was carried on Hypersil ODS C ls column （4. 6 mm × 250 mm, 5.0 μm） with acetonitrile and 3% acetic acid as the mobile phase in a gradient elution. The contents of 4 components were determined simultaneously. Results ： The fingerprint of 10 populations were established and the data were analyzed by the similari- ty evaluation software. There were almost no differences between the similarities of 10 population, but the contents of 4 main compoerls were different among them. Conclusion：This method is stable and reliable which could be applied in quality assessment.
出处
《中药材》
CAS
CSCD
北大核心
2012年第6期892-896,共5页
Journal of Chinese Medicinal Materials
基金
国家重大新药创制专项(No.2009ZX09103386)
关键词
金盏银盘
HPLC
指纹图谱
金丝桃苷
异槲皮苷
鬼针聚炔苷
紫云英苷
Bidens biternata （ Lour. ） Merr. et sherff.
HPLC
Fingerprint
Hyperoside
Isoquercetin
Astragalin
Bipinnatapolyacetylpside
