摘要
目的探讨四氧嘧啶（ALX）制作新西兰大白兔糖尿病心肌病模型的方法。方法选取健康新西兰大白兔30只，随机分为实验组（20只）和对照组（10只）。实验组经耳缘静脉注射5％ALX溶液，24h后再次注射，注射剂量均为80mg／kg；对照组注射等量的0．9％氯化钠注射液。分别监测两组兔注射后72h及2、4周的空腹血糖，并在饲养4周后分别左心室心肌行HE染色，观察心肌细胞病理变化。结果实验组14d内累计死亡8只．1只血糖恢复正常，成模率55％（11／20）；对照组无死亡。实验组成模兔注药后空腹血糖均较对照组兔明显增高，差异均有统计学意义（均P〈0．05）。4周后实验组兔心肌细胞排列不整齐，心肌细胞肿胀。间质纤维组织增多，心肌细胞浆空泡变性，炎细胞浸润。结论分次小剂量注射ALX制作糖尿病兔模型成功率较高，可建立稳定的糖尿病兔心肌病模型。
Objective To establish a diabetic cardiomyopathy rabbit model by injection of alloxan（ALX）. Methods Thirty healthy New Zealand white rabbits weighing 2.0-2.5kg were randomly divided into experimental group （n=20） and control group （n=10）. All rabbits were fasted for 12 hours,animals in experimental group were injected with 80mg/kg newly prepared 5% ALX, 24h later, injected the same dose again; animals in the control group were injected with same volume of normal saline. The fasting blood glucose levels were measured at 72h, 2w and 4w, respectively. The rabbits were sacrificed 4 weeks later, the pathological changes of left ventricular myocardium were observed under microscope. Results Eight rabbits in experiment group died in 14d and the fasting blood glucose of one rabbit returned to normal; the success rate of diabetic rabbit model was 55%（11/20）. There was no death in control group. Fasting blood glucose of experimental group was rising significantly compared with control group （P〈0.05）; the pathology showed vacuolar degeneration and necrosis in myocardiocytes and local inflammatory cells infiltration. Conclusion Multiple, low dose administration of ALX is effective to establish diabetic cardiomyopathy model in rabbits.
出处
《浙江医学》
CAS
2012年第15期1273-1275,共3页
Zhejiang Medical Journal
基金
浙江省自然科学基金资助项目（Y2110934）
