摘要
对11种热带植物的640 863条表达序列标签(ESTs)进行分析,发掘出13 916个EST-SSR位点,平均分布频率约为109.37个/Mb,主要特点表现为:(1)不同物种间的EST-SSR丰度存在明显差异,其中菠萝EST-SSR位点最为丰富(201.75个/Mb);芒果EST-SSR位点丰度最低(49.55个/Mb);(2)二、三核苷酸基元的SSR最为常见,两者占总数的88.68%。其中,AG/CT、AT/AT和AAG/CTT在不同基因组区域都是占优势的SSR类型;(3)SSR在基因组不同区域分布丰度存在很大差异。其中,麻疯树基因组SSR(gSSR)的丰度是EST-SSR丰度的17倍多;(4)保守基因内AG/CT重复的SSR极其丰富。此外,设计了一批具有物种特异性的EST-SSR PCR引物。
Simple sequence repeats （SSRs） were mined from 640 863 expressed sequence tags（ESTs） belonging to 11 tropical plant species. 13 916 EST-SSRs were detected with an average of 109.37/Mb. The main characteristics were as the following： （1） EST-SSR abundances were different between different tropical plant species. Pineapple tree had the most abundant EST-SSRs（201.75/Mb） while mango tree got the least（49.55/Mb）; （2） EST-SSRs with di-and tri-nucleotide motifs were the most abundant, which accounting for 88.68% of the total. Amongst which, AG/CT, AT/AT and AAG/CTT repeat motifs were predominant in all genomic regions; （3） SSR abundance were different in different genomic regions. The abundance of genomic SSRs（gSSRs） in Jatropha curcas was more than 17 times higher than that of EST-SSRs; （4） AG/CT repeat SSRs were extremely rich in conserved genes. Moreover, a set of species-specific EST-SSR PCR primers were designed.
出处
《热带作物学报》
CSCD
北大核心
2012年第7期1208-1214,共7页
Chinese Journal of Tropical Crops
基金
转基因新品种培育重大专项(No.2008ZX08012-04)
中国热带农业科学院中央级公益性科研院所基本科研业务费专项(No.ITBB110602)
海南省自然科学基金项目(No.312053)
