摘要
目的:观察采用双侧卵巢切除术制备的骨质疏松症模型大鼠的一般情况、生理状态,脏器湿重及血清学指标,以验证绝经后骨质疏松模型大鼠的肾虚证表现,为中医药临床防治骨质疏松症采用补肾治本的原则提供一定的理论基础。方法:24只雌性SD大鼠分为3组:模型组、假手术组和正常组。模型组麻醉后行双侧卵巢切除术,假手术组麻醉后行假手术处理,正常组进行麻醉处理。饲养3个月进行股骨的病理组织观察及骨密度检测,确认造模成功后进行大鼠的一般情况观察及生理状态的量化检测。麻醉下处死大鼠,用电子天平称垂体、肾、肾上腺和子宫的湿重,采用ELISA法进行ACTH、E2、T3、T4的血清学检测。结果:骨质疏松模型组大鼠出现体毛稀疏无光泽、畏寒倦卧、群聚角落、精神萎靡、眼睛黯淡、眼球运动慢、反应迟钝、活动迟缓、食欲下降、身体肥胖、嗜睡、大便略稀、小便清长等一系列肾虚证的表现;活动度、肛温及进食量均低于假手术组与正常组;血清ACTH、E2、T3、T4水平均低于假手术组与正常组;肾上腺、肾、子宫的湿重均低于假手术组与正常组。结论:采用经典的骨质疏松症造模方法即双侧卵巢切除术制备的骨质疏松症模型大鼠具有肾虚的器质性及功能性变化表现,骨质疏松症模型大鼠存在肾虚病机,具有肾虚的临床表现。
Objective ：To observe general conditions,physiological condition,viscera wet weight and kidney asthenia serological index in osteoporosis model rats established by bilateral ovarian resection, and provide basis for treating osteoporosis by invigorating kidney. Methods：Twenty-four female SD rats were randomly divided into 3 groups as model group, sham group and normal group. After anesthesia, model group were treated by bilateral ovarian resection, sham group by sham operation, and normal group without any processing. Pathological tissue and bone mineral density （BMD） of femur was performed 3 months postoperatively. The general conditions and physiological conditions were processed after successfully modeling. Elec- tronic balance was used to collect the weight of kidney, drenal,pituitary, and uterus. Estrogen （E2）, Adrenocortieotropie hor- mone （ACTH）, Three typical thyroid original acid （T3）, Four typical thyroid original acid （T4） were detected by ELISA. Re- suits： Osteoporosis model appeared with syndromes of kidney deficiency ,such as body hair without burnish ,chills ,cluster, sluggishness,eyes dark,eye movement slow,slow to respond to the delay,decreased appetite,activities slow,body fat,and drowsiness, defecate slightly thin andclear abundant urine. Mobility, anal temperature and food-intake were lower than normal and sham group; the level of ACTH, E2,T3, T4 lower than sham and normal group;the weight of kidney, drenal ,pituitary, and uterus also lower than other two groups. Conelusion：Osteoporotic model rats, established by bilateral ovarian resection, have manifestation of organic and functional performance of kidney deficiency. Therefore, nsteoporotie model rats have the pathogenesis of kidney asthenia and clinical manifestations of kidney deficiency.
出处
《中国骨伤》
CAS
2012年第9期766-770,共5页
China Journal of Orthopaedics and Traumatology
基金
卫生部科学研究基金(省部共建项目计划)(编号:WKJ2008-2-032)
浙江省中医药管理局(编号:2009YA001)
浙江省中医药重点研究项目计划(编号:2006Z003)
浙江省中药现代化专项项目[浙经信医化(2010)421号]~~
关键词
骨质疏松
肾虚
模型
动物
生理学
Osteoporosis
Kidney deficiency
Models,animal. Physiology
