摘要
目的:建立同时测定莱阳梨清膏中3个咖啡酰奎宁酸类成分3-O-咖啡酰奎宁酸、绿原酸(5-O-咖啡酰奎宁酸)、4-O-咖啡酰奎宁酸的HPLC方法。方法:采用Thermo BDS(4.6 mm×250 mm,5μm)色谱柱,以乙腈(A)-0.4%磷酸溶液(B)为流动相,梯度洗脱(0～35 min,5%A→10%A;35～40 min,10%A),流速1.0 mL.min-1,检测波长为327 nm。结果:3-O-咖啡酰奎宁酸、绿原酸(5-O-咖啡酰奎宁酸)、4-O-咖啡酰奎宁酸进样量分别在0.023～0.230,0.022～0.224,0.023～0.226μg范围内与峰面积呈良好的线性关系;平均回收率(n=6)分别为97.2%,97.0%,99.5%。结论:本法操作简便,结果准确,灵敏度高,可用于莱阳梨清膏中多指标成分的质量控制。
Objective：To develop an HPLC method for simultaneously determination of three caffeoylquinic acids （3 - O - caffeoylquinie acid, chlorogenic acid, 4 - O - caffeoylquinic acid） in Laiyang pear clear paste. Methods： Thermo BDS（4.6 mm ×250 mm,5μm）column was adopted. The mobile phase consisting of aeetonitrile（A） - 0. 4% phosphoric acid solution（B） with gradient elution （0 - 35 rain,5% A→10% A ;35 - 40 rain, 10% A） was ap- plied at a flow rate of 1.0 mL · min-1. The detection wavelength was 327 nm. Results：The calibration curves were linear in the ranges of 0. 023 -0. 230,0. 022 -0. 224,0. 023 -0. 226μg for 3 - 0 -caffeoylquinic acid, ehlorogen- ic acid（5 - 0 - eaffeoylquinie acid）, 4 - 0 - eaffeoylquinic acid; The average recoveries （ n = 6 ） were 97.2%, 97.0% and 99.5% ,respectively. Conclusion ：The method is simple, accurate, and sensitive ,which can be used for quality control of multi - component in Laiyang pear clear paste.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第9期1648-1650,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
高效液相色谱法
白梨
莱阳梨清膏
绿原酸
咖啡酰奎宁酸
异构体
中成药多指标成分检测
HPLC
Pyrus bretschneideri Rehd.
Laiyang pear clear paste
chlorogenic acid
caffeoylquinic acids
isomer
Chinese patent medicine multiple index component detection
