摘要
在饵料中添加0、500、3 000mg/kg的当归多糖(ASP)喂食点带石斑鱼(Epinephelus malabaricus)(初始体重(158.64±2.14)g),分别在喂食4和8周后取样,研究ASP对点带石斑鱼非特异性免疫力和抗病力的影响。结果表明,ASP能显著提高血液白细胞吞噬率和血清LZM活力(P<0.05),对血清一氧化氮(NO)、体表黏液溶菌酶(LZM)活力和抗菌活力无显著影响。ASP能促进头肾白细胞增殖、呼吸爆发活力和吞噬活力,降低用迟缓爱德华菌(Edwardsiella tar-da)攻毒后试验鱼的累积死亡率,3 000mg/kg组累积死亡率低于500mg/kg组,连续喂食ASP 8周的保护效果优于4周。研究表明,用含ASP 3 000mg/kg的饵料连续喂食点带石斑鱼8周能有效提高其非特异性免疫力和抗病力。
In order to study the effects of Angelica sinensis polysaccharide(ASP) on innate immunity and disease resistance of Epinephelus malabaricus,fish(initial body weight(158.64±2.14) g) were fed diets containing different doses of ASP(0,500,3000 mg/kg) for four and eight weeks.Results showed that dietary ASP enhanced blood leucocytes phagocytic rate and serum lysozyme activity significantly(P〈0.05),but exerted no influence on serum nitric oxide(NO),skin mucus lysozyme activity and antibacterial activity.Dietary ASP boosted head kidney leucocytes proliferation,respiratory burst activity and phagocytosis.The cumulative mortalities of fish challenged by Edwardsiella tarda were reduced in ASP-fed groups,compared to that in control groups.The cumulative mortality in fish fed with ASP at 3000 mg /kg for eight weeks was the lowest.The innate immunity and disease resistance against E.tarda in fish fed ASP at 3000 mg/kg for eight weeks were effectively enhanced.
出处
《中国海洋大学学报(自然科学版)》
CAS
CSCD
北大核心
2012年第9期15-21,共7页
Periodical of Ocean University of China
基金
"十二五"国家科技支撑计划项目(2011BAD13B07)资助
关键词
当归多糖
点带石斑鱼
非特异性免疫力
抗病力
Angelica sinensis polysaccharide
Epinephelus malabaricus
innate immunity
antibacterial activity