摘要
目的探讨盐诱导基因(Salt Inducible Kinase 2,SIK2)对3T3-L1成熟脂肪细胞生脂的影响及其机制。方法利用小鼠SIK2重组腺病毒表达载体,转染3T3-L1成熟脂肪细胞,采用油红O染色提取法和RT-PCR,测定脂肪细胞生脂及其关键酶和转录因子固醇调节元件结合蛋白1(SREBP1)mRNA水平的变化。结果与未转染组和空载体转染组相比,SIK2转染组油红O染色提取的脂滴含量降低56%(P<0.05),脂肪合成相关酶乙酰辅酶A羧化酶(ACC2)、脂肪酸合酶(FAS)、硬脂酰辅酶A去饱和酶-1(SCD-1)、甘油-3-磷酸转酰酶(GPAT)、二酰基甘油转酰酶1(DGAT1)的mRNA表达水平分别下降了31%、61%、57%、60%、52%(P<0.05),转录因子固醇调节元件结合蛋白1(SREBP1)mRNA的表达水平减少了28%(P<0.05)。结论 SIK2可抑制3T3-L1脂肪细胞脂肪合成代谢,其机制可能与下调甘油三脂合成代谢关键酶及转录因子SREBP1的基因表达有关。
Objective To study the biological function of SIK2（S alt Inducible Kinase 2） on lipogenesis in 3T3-L1 adipocytes and its mechanism. Methods 3T3-L1 adipocytes were infected by recombinant adenovirus vector conta ining mouse SIK2 gene.The lipogenesis and levels of lipogenic gene mRNAs in mat ure adipocytes were measured through Oil Red 0 staining and real-time PCR.The c hanges of transcription factor SREBP1 mRNA level were also determined by real-t ime PCR. Results In SIK2 over-expressing group,compared with the control group,mRNA levels of lipogenic genes such as acetyl CoA carboxylase（ACC2）,fatty acid synthase（FAS）, stearoyl CoA desaturease-1（SCD-1）,glycerol-3-phosphate acyltransferase（GPA T） and diacylglycerol acyltransferase1（DGAT1） were respectively significantly decre ased by 31%,61%,57%,60%,52%（P〈0.05）,which coincided with the reduction of lipogenesis in adipocytes.Sterol regulatory element binding protein（SREBP）-1 m R NA levels were coordinately reduced in SIK2 over-expressing adipocytes by 28%（ P〈O.05）. Conclusion Our results support that SIK2 may inhibit lipogenesis in a dipocyte energy metabolism by downregulation of lipogenic genes.Further study n eeds to be done in translation level.
出处
《中国实验诊断学》
2012年第8期1354-1357,共4页
Chinese Journal of Laboratory Diagnosis
基金
留学回国人员科研启动基金(2009)
深圳市科技计划项目(201002069)
