摘要
目的建立测定益阴降糖胶囊中葛根素含量的高效液相色谱法。方法色谱柱为Symmetry C18柱(250 mm×4.6 mm,5μm),以甲醇-水为流动相,流速为1.0 mL/min,检测波长为250 nm。结果葛根素进样量在0.108～1.08μg范围内与峰面积线性关系良好,加样回收率为98.40%,RSD为0.70%(n=9)。结论该法快速、准确,可用于该制剂的质量控制。
Objective To establish a HPLC method for the determination of puerarin in Yiyin Jiangtang Capsules. Methods The method was performed on a Symmetry C18 column（250 mm4.6 mm,5 μm） with a mobile phase of methanol-water at the flow rate of 1.0 mL/min and a 250 nm detection. Results The calibration curve of puerarin was linear in the range of 0. 108- 1.05 I^g, the averge recovery was 98.40%, RSD = 0.70% （n =9）. Conclusion The method is quick,accurate and can be used for quality control of Yiyin Jiangtang Capsules.
出处
《中国药业》
CAS
2012年第18期43-44,共2页
China Pharmaceuticals
