摘要
目的比较3种生物指示剂对压力蒸汽灭菌的监测效果。方法对菌片、自含式生物指示剂和快速阅读生物指示剂培养阳性进行对照,并在(121.0±0.5)℃条件下压力蒸汽灭菌5、10、20min后,观察培养结果。结果阳性对照组中菌片的阳性率为86.0%,自含式生物指示剂和快速阅读生物指示剂的阳性率均为100.0%;(121.0±0.5)℃条件下压力蒸汽灭菌20min,3种生物指示剂的阳性率均为0;灭菌10min,菌片的阳性率为0,自含式生物指示剂的阳性率为8.0%,快速阅读生物指示剂的阳性率为10.0%;灭菌5min,菌片的阳性率为82.0%,自含式生物指示剂和快速阅读生物指示剂的阳性率均为100.0%。结论自含式生物指示剂是一种价格适中、方便、快速、准确的监测压力蒸汽灭菌的方法,值得医院推广使用。
OBJECTIVE To compare the effects of three biological indicators on the surveillance of pressure steam sterilization.METHODS Three kinds of biological indicators cultured positive were set as the control study subjects,then the pressure steam sterilization was performed for 20min,10min,and 5min under the conditions of（121.0±0.5）℃,the results of the culture were observed.RESULTS The positive rate of the bacterial biofilm in the control group was 86.0%,the positive rates of both self-contained biological indicators and the biological indicators of rapid reading were 100.0%;the positive rates of the three biological indicators were 0 after the pressure steam sterilization for 20 min under the conditions of（121.0± 0.5）℃;the positive rate of the bacterial biofilm was 0 after sterilization for 10 min,the positive rate of self-contained biological indicators was 8.0%,and the positive rate of the biological indicators of rapid reading was 10.0%;the positive rate of the bacterial biofilm was 82.0% after sterilization for 5min,the positive rates of both self-contained biological indicators and the biological indicators of rapid reading were 100.0%.CONCLUSION Self-contained biological indicator is an affordable,convenient,fast and accurate method of monitoring steam sterilization,it is worth to promote in using of the hospital.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2012年第17期3803-3804,共2页
Chinese Journal of Nosocomiology
关键词
生物指示剂
压力蒸汽灭菌
生物监测
Biological indicators
Pressure steam sterilization
Biological monitoring
