摘要
运用甲醇、乙醇、NaOH、超声波4种方法对哈茨木霉分生孢子进行提取,并将提取物分别对杨树烂皮病菌、水稻稻瘟病菌进行了抑菌试验。对杨树烂皮病菌的抑菌试验结果表明:超声波水法中D1提取物稀释100倍的抑菌率最高,为43.42%;3种超声波水法提取物抑菌率的方差分析表明,各处理差异显著;将NaOH提取物稀释100倍后抑菌率为39.46%,而甲醇和乙醇的提取物的抑菌效果较弱,分别为10.60%和13.44%。对水稻稻瘟病菌1号、2号菌株的抑菌试验表明:D1提取物稀释100倍的抑菌率均较高,分别为42.18%和31.99%;将甲醇提取物稀释100倍后对1号菌株的抑菌率为35.78%;将乙醇提取物稀释100倍后,对2号菌株的抑菌率为18.52%。
Four extraction methods of methanol, ethanol, NaOH and ultrasonic were applied to extract the conidia of Trichoderma harzianum. Bacteriostatic tests with conidial extracts of T. harzianum against Cytospora chrysosperma and Magnaporthe grisea were performed. Results show that the first ultrasonic extract diluted 100-fold had the highest inhibition rate （43.42%）. The variance analysis of inhibition rate of ultrasonic extract indicated a significant difference between different treatments. The inhibition rate of the 100-fold diluted extracts by NaOH was 39.46%. However, the inhibition effects of the extracts by methanol or ethanol were weak, and the inhibition rates were 10. 60% and 13.44%, respectively. The bacteriostatic test against strains No. 1 and No.2 of rice blast fungus （M. grisea） showed that the first extract diluted 100-fold had the highest inhibition rate, which were 42.18% and 31.99%, respectively. The inhibition rate of the 100-fold diluted extract by methanol to strain No. 1 was 35.78%, and that of the 100-fold diluted extract by ethanol to strain No. 2 was 18.52%
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2012年第9期90-92,106,共4页
Journal of Northeast Forestry University
基金
黑龙江省自然科学基金重点项目(ZD200901)
