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拟南芥类锌指基因AT3G02790/AT5G16470纯合双突变的筛选及其表型分析 被引量：2

Acquisition of Zinc Finger Gene AT3G02790/AT5G16470 Double-Mutant of Arabidopsis thaliana and Characterization of Mutant Phenotype
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摘要 以拟南芥T-DNA插入突变体AT3G02790和AT5G16470为亲本,通过人工杂交技术获得杂合体后,结合基因分离定律和PCR技术筛选出拟南芥纯合双突变株,并对拟南芥纯合突变体的表型进行了初步分析,结果显示:突变体与野生型植株表型无明显差异,推测该基因为功能未知的新基因。 In this research, zinc finger gene mutants were crossed with T-DNA insertion in AT3G02790 and ATSG16470 to pro- duce A13GO2790/ATSG16470 double-mutant. PCR technique and law of gene segregation were used to identify homozygous double-mutant. In addition, the phenotype of the homozygous double-mutant was analyzed. Results showed a similar growth performance with wild-type plants. It is specttlated that AT3G02790 and AT5G16470 are genes with unknown function.
作者 郑唐春 施晓文 臧丽娜 朱银凤 王亚军 曲冠证
机构地区 林木遗传育种国家重点实验室(东北林业大学) 吉林省林业勘察设计研究院
出处 《东北林业大学学报》 CAS CSCD 北大核心 2012年第9期101-106,共6页 Journal of Northeast Forestry University
基金 国家自然科学基金项目(31070595)资助
关键词 拟南芥 纯合双突变 杂交 表型 胁迫 Arabidopsis thaliana Double-mutant Crossing Phenotypes Stress
分类号 Q753 [生物学—分子生物学]
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共引文献60

同被引文献45

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东北林业大学学报
2012年 第9期

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