摘要
目的建立HPLC法同时测量安博诺片中厄贝沙坦和氢氯噻嗪的含量。方法安博诺片中厄贝沙坦和氢氯噻嗪的HPLC同时测定方法:采用Venusil XBP-C18色谱柱(4.6 mm×250 mm,5μm),流动相是乙腈-0.1 mol.L-1磷酸盐溶液(加入0.1%三乙胺,磷酸调pH值5.0)(60∶40),流速1.0 mL.min-1,检测波长260 nm,进样量为20μL。结果安博诺片中厄贝沙坦和氢氯噻嗪的HPLC同时测定方法:厄贝沙坦和氢氯噻嗪分别在1.5～30μg.mL-1和1.2～25μg.mL-1范围内线性关系良好;平均回收率(n=9)分别为100.4%和100.3%;精密度实验RSD分别为0.23%和0.15%;供试品在12小时内稳定。结论建立的方法操作简便,测量结果准确、可靠。
Objective To determine the content of irbesartan and hydrochlorothiazide in Anbonuo compound tablets by high performance liquid chromatography （HPLC）. Methods Simultaneous determination by HPLC： A Venusil XBP - C18 column （250 mm ×4. 6 mm, 5 um） was used, with mobile phase of acetonitrile （ -0. 1 mol. L-1 ） and phosphate solution （ pH 5.0） （ 60 ： 40）, containing 0. 1% triethylamine. The flow rate of the mobile phase was 1.0 mL .min -1 , the detection wavelength was 260 nm and the injection volume was 20uL. Results By HPLC, the calibration curves of irbesartan and hydrochlorothiazide showed a good linear relationship at the range of 1.5 - 30 ug. mL - 1 and 1.2 - 25 ug . mL-1. Average recovery were 100.4% and 100. 3% respectively, and the relative standard derivation （RSD） of precision experiment were 0. 23% and 0. 15%. The sample solutions for test were stable in 12hours. Conclusion HPLC is simple to operate and the results are accurate and reliable.
出处
《辽宁医学院学报》
CAS
2012年第4期338-340,共3页
Journal of Liaoning Medical University (LNMU) Bimonthly
