摘要
目的:观察蛭元方对血小板加剧的类风湿关节炎(rheumatoid arthritis,RA)大鼠滑膜细胞炎症和增生反应的影响。方法:将常规培养的大鼠类纤维母样滑膜细胞株RSC364随机分为正常对照组、RA模型组、活化血小板干预组和蛭元方干预组。在不同浓度TNF-α诱发的大鼠RA滑膜细胞模型的基础上,以牛II型胶原蛋白刺激血小板活化,用蛭元方含药血清作用于活化血小板干预下的RA模型细胞,采用酶联免疫吸附试验(ELISA)法分别检测RSC364细胞培养上清中白细胞介素-6(IL-6)、白细胞介素-8(IL-8)的生成量和P-选择素的表达情况,采用MTT比色法观察RSC364细胞增殖的情况。结果:①牛II型胶原蛋白活化血小板5 h时,血小板表达P-选择素最多;②在不同浓度肿瘤坏死因子-α(TNF-α)存在下,RA模型组RSC364细胞培养上清中IL-6和IL-8的生成量均增加,与同一浓度的正常对照组相比均有显著性差异(P<0.05);RSC364细胞增殖率也明显高于正常对照组(P<0.05),并呈现剂量依赖性;③与RA模型组相比,活化血小板干预组RSC364细胞生成IL-6和IL-8的量均明显增加(P<0.05和P<0.01),滑膜细胞增殖率显著升高(P<0.05);④与血小板干预组相比,蛭元方干预组RSC364细胞的IL-6和IL-8生成量均显著降低(P<0.05和P<0.01),增殖率亦有明显下降(P<0.05),同时P-选择素的表达量显著降低(P<0.05)。结论:活化血小板能加剧RA滑膜细胞的炎症和增生反应;蛭元方能有效缓解血小板对RA大鼠滑膜炎症及增生的促进作用,其机制可能与抑制P-选择素的表达有关。
Objective：To observe the effects of Zhiyuanfang inhibiting platelets amplified inflammation and proliferation of synovial cells in the rheumatoid arthritis（RA） rats.Method：Normal cultured RSC364 cells were divided into normal control group,RA model group,activated platelet intervention group and Zhiyuanfang group.The different concentrations of tumor necrosis factor-α（TNF-α） were used to induce RA rat cell models,stimulate platelets activating by bovine type II collagen,and interfere platelet intervention group with Zhiyuanfang containing serum.The expression of interleukin-6（IL-6）,interleukin-8（IL-8） and P-selecting were assayed respectively by enzyme-linked immunosorbnent assay（ELISA）,and the proliferation of RSC364 cells were determined by MTT colorimetric assay.Result：①The expression of P-selecting was at peak when platelets were activated by bovine type II collagen for 5 h.②the expression of IL-6 and IL-8 on synovial cells in RA model group was increased,which was compared with the same concentration of normal group showed a significant difference（P〈0.05）;the rate of RSC364 cells proliferation was also higher than normal group（P〈0.05）in a dose-dependent manner.③Compared with RA model group,the expression of IL-6 and IL-8 on synovial cells in platelets intervention group was significantly increased（P〈0.05,P〈0.01）,and the rate of synovial cells proliferation was also higher（P〈0.05）.④ Compared with platelets intervention group,the expression of IL-6 and IL-8 on synovial cells in Zhiyuanfang group was significantly reduced（P〈0.05,P〈0.01）,the proliferation rate of synovial cells was declined（P〈0.05）,and the expression of P-selecting was also lower.Conclusion：Activated platelets amplify the inflammation and proliferation of synoviocytes;Zhiyuanfang effectively can relieve the amplification of platelets on the inflammation and proliferation in RA rats probably by the inhibition of P-selecting expression.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第18期182-186,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
"十一五"全军中医药重大临床攻关课题(2006011003)
河北省医学科学研究重点课题(20100450)
河北大学医学部博士研究基金(200906001)
