摘要
目的:研究弯管列当不同提取部位对DPPH(1,1-二苯基苦基苯肼)自由基的清除能力及对H2O2诱导的人胚肾细胞系HEK-293细胞氧化损伤的保护作用。方法:弯管列当70%乙醇粗提物,依次用乙酸乙酯、正丁醇萃取后,得到乙酸乙酯提取部位(gg1)、正丁醇提取部位(gg2)和水部位(gg3)。用清除DPPH自由基法测试gg1,gg2,gg3的抗氧化活性。用H2O2建立人胚肾细胞系HEK-293细胞氧化损伤模型,用MTT法检测细胞活力,观察gg1,gg2,gg3对氧化受损细胞活力的影响。结果:DPPH实验的IC50从小到大依次为:gg1(0.042 9 g.L-1)<gg2(0.059 9 g.L-1)<VC(0.071 8 g.L-1)<gg3(0.330 3 g.L-1),表明gg1,gg2具有较强的抗氧化活性。研究结果亦表明弯管列当的gg1,gg2,gg3均能明显提高受损伤HEK-293细胞的活力,表现出抗氧化活性。结论:弯管列当以上各提取部位均具有一定的清除自由基、抗氧化的活性。
Objective：To investigate 1,1-diphenyl-2-picrylhydrazy1（DPPH） free radical scavenging activities of extracts from Orobanche cernua var.cumana.Method：The 70% ethanol extracts from O.cernua var.cumana was extracted successively with ethyl acetate and n-butanol.The three fractions of ethyl acetate,n-butanol,and water were tested using assays of the scavenging effects on DPPH.The model of oxidative damage of human embryonic kidney cell line HEK-293 cells was induced by H2O2,MTT assay was used to evaluate the cell viability.Result：The IC50 in DPPH test were in the following orders：ethyl acetate（0.042 9 g · L-1）〈n-butanol（0.059 9 g · L-1）〈VC（0.071 8 g · L-1）〈water（0.330 3 g · L-1）.The ethyl acetate fraction showed the strongest scavenging activity to DPPH free radicals.The three fractions of ethyl acetate,n-butanol and water could significantly improve the survival rate of injured HEK-293 cells,showing the antioxidant activities.Conclusion：These results demonstrate that the extracts from O.cernua var.cumana have some anti-oxidant and free radical scavenging activities.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第18期232-235,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
内蒙古自治区自然科学基金项目(2010BS1202)
内蒙古医学院科学技术研究项目博士启动基金(NY2010BQ007)
