摘要
目的通过测定血浆色氨酸(TRP)、犬尿氨酸(KYN)和犬尿喹啉酸(KYNA)浓度,探讨外周吲哚胺2,3-双加氧酶(IDO)和犬尿氨酸氨基转移酶(KAT)活性与原发性高血压(EH)的关系。方法采用高效液相色谱法(HPLC)测定100例EH患者和80名健康体检者(对照组)血浆TRP、KYN和KYNA浓度,计算产物/底物的百分比来评价酶活性,即IDO活性=KYN/TRP×100%,KAT活性=KYNA/KYN×100%。结果 EH组血浆TRP浓度为(59.85±9.89)μmol/L,明显高于对照组[(48.19±7.72)μmol/L,P<0.001];KYN浓度为(2.01±0.48)μmol/L,明显低于对照组[(2.17±0.43)μmol/L,P<0.05]。EH组和对照组血浆KYNA浓度分别为(24.10±9.12)、(23.59±7.27)μmol/L,二者差异无统计学意义(P>0.05)。EH组IDO活性为3.40%±0.85%,明显低于对照组(4.54%±0.81%,P<0.001);而KAT活性为1.20%±0.36%,明显高于对照组(1.09%±0.27%,P<0.05)。EH组血浆TRP浓度与年龄呈负相关(r=-0.316,P=0.001),而对照组二者之间无明显相关性(r=-0.208,P=0.064)。EH组和对照组血浆IDO活性均与年龄呈正相关(EH组:r=0.264,P=0.008;对照组:r=0.305,P=0.006)。2组血浆KYN、KYNA浓度及KAT活性与年龄均无明显相关性。结论EH组血浆TRP浓度明显增高而KYN浓度明显降低,提示外周TRP-KYN代谢途径的IDO活性可能与EH有关。
Objective To investigate the correlations between the pathogenesis of essential hypertension（EH） with peripheral indoleamine 2,3-dioxygenase（IDO） and kynurenine aminotransferase（KAT） activities by measuring plasma concentrations of tryptophan（TRP）,kynurenine（KYN） and kynurenic acid（KYNA）.Methods TRP,KYN and KYNA were measured by high performance liquid chromatography（HPLC） in the plasma of 100 patients with EH and 80 healthy controls.The enzyme activity was estimated by calculating the percentage of product/substrate,as IDO activity=KYN/TRP×100% and KAT activity=KYNA/KYN×100%.Results TRP concentrations were（59.85±9.89） μmol/L,which were significantly higher in patients with EH than in healthy controls [（48.19±7.72） μmol/L,P〈0.001].KYN concentrations were（2.01±0.48） μmol/L,which were significantly lower in patients with EH than in healthy controls [（2.17±0.43） μmol/L,P〈0.05].The concentrations of KYNA in the patients with EH were（24.10±9.12） μmol/L,and were（23.59±7.27） μmol/L in healthy controls,and there were no statistical significances between the 2 groups（P〉0.05）.The IDO activity was 3.40%±0.85%,which was significantly lower in the patients with EH than in healthy controls（4.54%±0.81%,P〈0.001）.KAT activity（1.20%±0.36%） in patients with EH was significantly higher than that in healthy controls（1.09%±0.27%,P〈0.05）.Plasma TRP concentration in the patients with EH had negative correlation with age（r=-0.316,P=0.001）,and there was no correlation in the healthy controls（r=-0.208,P=0.064）.Plasma IDO activity in the patients with EH and the healthy controls had positive correlation with age（EH group： r=0.264,P=0.008;control group： r=0.305,P=0.006）.There was no obvious correlation with age between the 2 groups.Conclusions The increased concentrations of TRP and decreased concentrations of KYN in plasma of EH patients suggest that peripheral IDO activity in the TRP-KYN metabolism pathway may be involved in the pathogenesis of EH.
出处
《检验医学》
CAS
2012年第8期624-627,共4页
Laboratory Medicine
基金
上海市卫生局科研项目(2009206)
