摘要
为建立一个大豆子叶节高效再生体系用于大豆的遗传转化,以桂春豆1号、桂早2号、桂夏1号和桂夏豆2号4个大豆品种为材料,子叶节为外植体诱导丛生芽,再生完整植株。实验研究了大豆种子的消毒方法,基因型以及培养过程中植物激素的浓度等影响大豆子叶节再生的因素。结果表明在适宜的条件下,4个大豆品种的丛生芽分化率都可达到90%,丛生芽数基本都在4～6个范围内,4个大豆品种均为子叶节器官发生途径的理想基因型。最适合的种子灭菌方法是双氧水灭菌法;桂春豆1号和桂早2号的适宜芽诱导培养基为B5+6-BA 1.6 mg/L+IBA 0.2 mg/L,桂夏1号和桂夏豆2号为B5+6-BA 1.0 mg/L+IBA 0.2 mg/L;4个大豆品种的适宜丛生芽伸长培养基为1/2 MS+B5有机+GA30.5 mg/L;生根培养基为B5+NAA 0.5 mg/L+2.0 g/L活性炭。
To establish a high frequency regeneration system for the gene transformation of soybean,seed disinfection,concentration of auxin in three processes（germination medium,differentiation of multiple shoot and rooting） during soybean cotyledonary node regeneration was studied by using GCD1,GZ2,GX1,GXD2.The results show that all the genotypes used in the test produced the highest regeneration rate,up to 90 %;the range of multiple shoots between 4-6;all the genotypes can be used.The most suitable soybean seed sterilization was H2O2,and the optimal concentration of 6-BA and IBA in multiple shoots induction medium was 1.6 and 0.2 mg/L for GCD1 and GZ2;0.2 mg/L IBA and 1.0mg/L 6-BA for GX1 and GXD2.The optimum culture medium for elongation of adventitious bud was 1/2MS＋ B5 organism ＋ 0.5 mg/L GA3,and the optimum culture medium for rooting was B5＋ 0.5 mg/L NAA ＋ 2.0 g/L.
出处
《西南农业学报》
CSCD
北大核心
2012年第4期1181-1186,共6页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(30871545)
广西回国基金项目(桂科回0832020)
广西农业科学院基本业务费项目(201004Z(基))
广西农业科学院基本科研业务专项(2012YM23)
关键词
大豆
子叶节
丛生芽
植株再生
Soybean
Cotyledonary node
Multiple shoots
Plant regeneration
