摘要
目的探讨Wnt5amRNA和蛋白、结肠腺瘤性息肉病基因（APC）和B-连环素（B—catenin）蛋白在结直肠腺癌组织中的表达及意义。方法应用实时定量PCR法，检测30例新鲜结直肠腺癌组织和配对的痛旁组织中Wnt5amRNA的表达。采用免疫组织化学sP法，检测62例结直肠腺癌和癌旁组织以及10例正常结直肠黏膜组织中Wnt5a、APC和B—catenin蛋白的表达情况，并分析Wnt5a、APC和B．catenin蛋白的表达与结直肠腺癌患者临床病理特征的关系。结果Wnt5amRNA在新鲜结直肠腺癌组织中的相对表达量为0．1232±0．0140，明显高于其在癌旁结直肠黏膜组织中的相对表达量（0．0497±0．0074，P=0．02）。Wnt5a蛋白在61．3％（38／62）的结直肠腺癌组织中低表达，在38．7％（24／62）的结直肠腺癌组织中高表达，其表达与肿瘤的组织学类型和分化程度有关（均P〈0．05）。APC蛋白住61．3％（38／62）的结直肠腺癌组织中低表达，其表达与肿瘤的组织学分犁有关（P〈0．05）。B—catenin蛋白在80．6％（50／62）的结直肠腺癌组织中呈胞质和（或）胞核的异位表达，其表达与肿瘤的分化程度、有无淋巴结转移、浸润深度以及TNM分期均有关（均P〈0．05）。在结直肠腺癌组织中，Wnt5a（r=0．271，P=0．027）和APC（r=0．343，P=0．004）蛋白的低表达均与B—catenin蛋白的异位表达相关，仉Wnt5a与APC蛋白的表达无关（r=0．218，P=0．078）。结论Wnt5a、APC和B—catenin基因可能参与了结直肠腺癌的发生和发展过程。在结直肠腺癌中，APC和Wnt5a蛋白的低表达可能是B-catenin蛋白异位表达的原因之一。
Objective To study the expression of WntSa gene mRNA and WntSa, APC, 13-catenin proteins in human colorectal adenocarcinoma （CRC） and explore its clinical significance. Methods WntSa mRNA level was measured in 30 patients with CRC and paired non-tumor tissues by real-time PCR. Immunohistochemical staining of WntSa, APC, β--catenin was performed in samples of 62 patients with CRC using SP system. Results The relative expression level of WntSa mRNA in fresh CRC is 0. 1232±0.0140, which is significantly higher than that in adjacent colorectal mucosa（0. 0497±0. 0074, P = 0.02）. A low expression of WntSa protein was observed in 38 of 62 CRC. WntSa protein expression was closely correlated with the tumor types and the degree of tumor differentiation （ P 〈 0.05 ）. There was no apparent relationship with lymph node metastasis, depth of myometrial invasion and TNM stages （P 〉 0.05）. APC protein was decreased in 38 of 62 CRC. The expression of APC was closely correlated with the tumor types （P 〈 0.05 ）. There was no apparent relationship with the degree of tumor differentiation, lymph node metastasis, depth of myometrial invasion and TNM stages （ P 〉 0.05 ）. The expression of β--catenin was observed in cytoplasm and/or cell nuclei in 50 of 62 CRC. The positive rate of β--catenin expression was closely correlated with the degree of tumor differentiation, lymph node metastasis, depth of myometrial invasion and TNM stages （ P 〈 0.05 ）. There was no apparent relationship with the tumor types （ P 〉 0.05 ）. The expressions of Wnt5 a （ r = 0.271, P = 0. 027 ） and APC （ r = 0. 343, P = 0.004 ） were correlated with that of β--catenin in CRC, respectively, but there was no correlation between the expressions of WntSa and APC protein （r = 0.218, P = 0.078）in the tumors. Conclusions WntSa, APC and β-catenin genes might be involved in the carcinogenesis and development of CRC. It is hypothesized that down-regulation of APC and WntSa proteins may be one of causes of ectopic expression of β-catenin in CRC.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2012年第9期674-678,共5页
Chinese Journal of Oncology
