摘要
目的观察并探讨转化生长因子β1(transforming growth factorβ1,TGF-β1)在退变椎间盘组织中的表达与新生血管的关系及其临床意义。方法采用免疫组化SP法,检测38例腰椎间盘突出症患者手术切取的椎间盘组织,与10例正常椎间盘组织中TGF-β1及CD34的表达情况。结果 TGF-β1和CD34在退变椎间盘中的阳性率分别为65.8%(25/38)和63.2%(24/38),在正常椎间盘组织中均未见表达(P<0.05)。TGF-β1和CD34的表达与患者的性别和年龄无关(P>0.05)。TGF-β1和CD34在病程3年以上患者的阳性表达明显高于3年以内者(P<0.05)。TGF-β1和CD34在脱出型和游离型的阳性表达比突出型更为明显(P<0.05)。TGF-β1和CD34阳性率呈正相关(P<0.05)。结论 TGF-β1和CD34在退变椎间盘组织的表达均明显升高,且两者之间的表达具有相关性,证实其在椎间盘退变的发病机制中可能具有一定的作用。
Purpose To observe angiogenesis and the expression of TGF-β1 and discuss its significance in normal and degenerated lumbar intervertebral dises. Methods SP immunohistoehemieal method was used to detect the expression of TGF-β1 and CD34 in 38 tissue samples surgically removed from patients with herniated discs and 10 normal tissue samples. Results The positive rates of TGF- β1 and CD34 in degenerated lumbar intervertebral discs were 65.8% （25/38） and 63.2% （24/38） , there were no expression in nor- real tissue samples （ P 〈 0. 05 ）. There was no direct relationship between the patients＇ age and the positive rate of expression of TGF- β1 and CD34 （P 〉0. 05）. The positive rate of expression of TGF-β1 and CD34 in those whose course longer than 3 years was higher than that with a shorter course of illness of less than 3 years （P 〈 0. 05）. The positive rate of expression of TGF-β1 and CD34 in extru- sion type and separation type was higher than protruded type （ P 〈 0.05）. A positive eorrelation between the positive rate of expression of TGF-β1 and CD34 （P 〈 O. 05 ） was observed. Conclusions The expression of both TGF-β1 and CD34 increases in degenerated lumbar intervertebral discs and there is significant eorrelation between them, which thereby suggests that they may contribute to process of intervertebral disc degeneration.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2012年第9期983-986,共4页
Chinese Journal of Clinical and Experimental Pathology
