摘要
为了探讨三疣梭子蟹对不同盐度水平的生理响应以及成熟前后的生理变化,实验采用水化学和酶学方法,研究了盐度(15、20、25、30和35)对成熟前后三疣梭子蟹耗氧率、排氨率、氧氮比(O∶N)以及己糖激酶(HK)、丙酮酸激酶(PK)、琥珀酸脱氢酶(SDH)和乳酸脱氢酶(LDH)4种呼吸代谢酶活力的影响。结果发现:1.盐度与规格的交互作用对三疣梭子蟹呼吸代谢无显著影响(P>0.05);2.盐度对三疣梭子蟹耗氧率的影响不显著(P>0.05),但显著影响其排氨率(P<0.05);成熟前后的三疣梭子蟹耗氧率和排氨率差异显著(P<0.05);3.盐度对三疣梭子蟹O∶N未产生显著影响(P>0.05),三疣梭子蟹成熟前O∶N显著高于成熟后(P<0.05);4.除LDH外,盐度对三疣梭子蟹呼吸代谢酶活力影响不显著(P>0.05);除鳃中SDH和LDH外,三疣梭子蟹呼吸代谢酶活力随生长发育发生显著变化(P<0.05)。研究表明,规格对三疣梭子蟹呼吸代谢的影响高于盐度,长时间盐度驯化可能减弱甚至消除三疣梭子蟹在不同盐度水平下代谢的差异。
To evaluate the physiological responses and changes of swimming crab,Portunus trituberculatus between pre- and post-maturity at different salinities （ 15,20,25,30 and 35 ）, the oxygen consumption rate （OCR）, ammonia excretion rate （AER）and respiratory enzyme activities including hexokinase （HK）, pyruvate kinase（PK）, succinate dehydrogenase （SDH）and lactate dehydrogenase （LDH）were measured in this study. Results showed that the interaction of salinity and size did not influence the respiratory metabolism significantly. Salinities had a significant effect on AER other than OCR. Marked differences were found in both OCR and AER between pre- and post-maturity individuals（ P 〈 0.05 ）. No significant difference of O： N ratio was found among different salinities（P 〉0.05）,however,O： N ratio in pre-maturity individuals was significantly higher than that in post-maturity individuals（P 〈 0.05 ）. It was noticed that the activity of HK and PK changed significantly with size rather than salinity （ P 〈 0. 05 ）. Excluding LDH, no significant correlation between respiratory metabolism enzymes and salinity was observed （ P 〉 0.05）. However, the respiratory enzymes in tested tissues changed significantly between pre- and post-maturity （P 〈 0.05） except SDH and LDH in gills（P 〉 0.05 ）. The study indicated that compared to salinity, body size more impacted the respiratory metabolism of P. trituberculatus. And it is suggested that long-term salinity acclimatization could lower or eliminate the difference of respiratory metabolism of P. trituberculatus at different salinities.
出处
《水产学报》
CAS
CSCD
北大核心
2012年第9期1392-1399,共8页
Journal of Fisheries of China
基金
国家科技支撑计划(2011BAD13B03)
关键词
三疣梭子蟹
盐度
成熟前后
呼吸代谢
Portunus trituberculatus
salinity
pre- and post-maturity
respiratory metabolism
