摘要
目的:探讨参附注射液对大鼠全脑缺血/再灌注损伤的保护作用及其机制。方法:将SD雄性大鼠40只,随机分为4组(n=10):假手术组、模型对照组、尼莫地平组(30 mg/kg)和参附注射液组(10 mg/kg)。采用Pulsinelli’s四动脉阻断法造成全脑缺血/再灌注损伤模型(CI/R),分别于手术前1 d,术前1 h和再灌注前30 min给药,共3次。分别用高效液相色谱法测定脑组织谷氨酸(Glu)、天冬氨酸(Asp)和甘氨酸(Gly)含量,原子吸收分光光度测定Ca2+含量,干湿重法测定脑组织含水量,化学比色法测定脑组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:与假手术组比较,CI/R模型组大鼠脑组织Glu、Ca2+、MDA含量和含水量明显升高(P<0.05,P<0.01),SOD活性明显降低(P<0.05);参附注射液能显著降低脑组织Glu、Ca2+含量和含水量(P<0.05,P<0.01),显著升高SOD活性及SOD/MAD比值(P<0.05)。结论:参附注射液防治脑缺血/再灌注损伤的机制与降低兴奋性氨基酸(EAA)毒性、阻滞Ca2+超载和提高抗氧化能力有关。
Objective： To study the the protective effects and mechenism of Shenfu injection on the global cerebral ischemia /reperfusion（I/R） injury of rats.Methods： Divided 40 SD male rats into 4 groups randomly（n=10）：sham operation group,model control group,nimodipine group（30 mg/kg） and Shenfu injection group（10 mg/kg）.Made global cerebral ischemia/reperfusion injury model（CI/R） by adopting Pulsinelli’s Four Arterial Acclusion method,and then practised administration three times,respectively one day before the surgery,one hour before the surgery and 30 minutes before reperfusion.Finally,measured the contents of brain tissue glutamate（Glu）,aspartic acid（Asp） and Glycine（Gly） by means of High Performance Liquid Chromatography,the content of Ca2＋ by means of Atomic absorption spectrophotometry,the brain water content by means of wet and dry weight,the activity of brain tissue superoxide dismutase（SOD） and content of malondialdehyde（MDA） by means of chemical colorimetry.Results： Compared with the sham operation group,the contents of Glu、Ca2＋、MDA and water in the brains of CI/R model group rats increased remakably（P〈0.05 or P〈0.01）,while the activity of SOD decreased apparently（P〈0.05）;Shenfu injection could significantly decrease the contents of Glu、Ca2＋ and water（P〈0.05,P〈0.01）in brain tissue and remarkably increased the activity if SOD and the ration of SOD/MDA（P〈0.05）.Conclusion： The mechenism of Shenfu injection preventing cerebral ischemia/reperfusion injury is relevant to excitatory amino acid toxicity reduction,Ca2＋ overload blockage and antioxidant capacity improvement.
出处
《中国应用生理学杂志》
CAS
CSCD
2012年第5期462-465,共4页
Chinese Journal of Applied Physiology
基金
湖北省教育厅科技创新项目(J200529001)
