摘要
目的:研究miR-183在人乳腺癌组织和细胞株中表达状况及其对乳腺癌细胞生物学行为的影响。方法:使用实时定量PCR方法检测miR-183在人乳腺细胞株和临床乳腺病变组织中表达;使用脂质体介导的miRNA转染技术,检测转染miR-183后对乳腺癌细胞增殖、侵袭和迁移活力的影响。结果:高度侵袭力的乳腺癌细胞株miR-183表达较低侵袭乳腺细胞株显著下调(P<0.01);临床人乳腺癌组织中miR-183表达较乳腺良性病变组织显著下调(P<0.01);使用miR-183抑制物和模拟物分别转染乳腺癌细胞株MCF-7和MDA-MB-231后,乳腺癌细胞侵袭和迁移活力分别上调和下调(P<0.05),而细胞增殖活力改变不明显(P>0.05)。结论:乳腺癌中miR-183表达出现失调。miR-183可能参与了乳腺癌细胞侵袭和迁移过程。
AIM： To investigate the expression of miR - 183 in breast cell lines and tissue specimens and its effects on the biological behaviors of breast cancer cells. METHODS： Human breast cell lines and clinical tissue speci- mens of breast diseases were used in the study. The expression of miR - 183 was determined by real - time PCR. The char- acteristics of cell proliferation, invasion and migration were examined after miR- 183 was transfected by lipofection. RE- SULTS： Altered expression of miR - 183 was found in the highly invasive breast cancer cells as compared with weakly in- vasive breast cells. The expression of miR - 183 was also significantly decreased in the breast cancer tissues as compared with that in the benign breast disease tissues. The capacities of breast cancer cell invasion and migration were significantly increased after transfection of miR - 183 inhibitor and were decreased after transfection of miR - 183 mimic. No significant change of cell proliferation was observed after miR- 183 transfection. CONCLUSION： miR- 183 may play an important role in breast cancer progression, especially in the cell invasion and migration.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2012年第8期1358-1361,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81172533
No.30873047
No.81101597)
