摘要
目的研究基质细胞衍生因子-1（SDF-1）在视网膜培养中促神经节细胞轴突再生的作用。设计实验研究。研究对象Fischer 344大鼠视网膜。方法视网膜培养之前5天夹断大鼠视神经;培养前用多聚赖氨酸及层黏连蛋白包被培养板;培养时,把分离出来的视网膜放射状剪成8小片,每小片视网膜粘铺于已包被好的培养板孔中,每5-6块取自不同大鼠的视网膜块随机组成一组,共7组,分别置于7种不同的Neurobasal-A/B27培养基中（对照组、20 ng/ml、70 ng/ml、200 ng/ml、500ng/ml及1000 ng/ml5个SDF-1浓度梯度处理组及SDF-1联合AMD3100阻断组）培养7天,在倒置显微镜下计数再生神经轴突的数量及长度,并对各组进行比较。主要指标再生神经轴突的数量及长度。结果对照组平均每个视网膜培养块的再生神经突数量及长度分别为17根及约1 mm;SDF-1促进轴突再生的作用随其浓度的增高而增强,中高浓度SDF-1组可使轴突再生增强2-3倍,而应用受体阻断剂AMD3100可使SDF-1的促再生作用减半。结论 SDF-1具有促进视网膜神经节细胞轴突再生的作用且具有浓度依赖性。
Objective To investigate whether stromal cell-derived factor-1 （SDF-1） has promoting effects on axonal regeneration in retinal explant. Design Experimental study. Participants Fischer 344 rats retina. Methods Optic nerve of rats were crushed 5 days be- fore retinal explant culture, and culture plate was coated with poly-lysine and laminin before culture. Retinas were dissected out from eye cups and cut into 8 pieces followed by sticking onto the coated wells with retinal ganglion cell layer downward. Retinal pieces were incubated in neurobasal-A/B27 for 7 days before examination under an inverted fluorescent microscope. Explants were assigned to the following groups： control, SDF-1 groups with 20 ng/ml, 70 ng/ml, 200 ng/ml, 500ng/ml, 1000 ng/ml, and SDF-1 plus AMD3100 group. Main Outcome Measures Number and average length of regenerating axons. Results Average umber and length of the regenerating ax- ons in control group were 17/explant and lmm respectively. Promoting effects of SDF-1 was positively correlated to its concentrations. SDF-1 with middle and high concentration enhanced the axonal regeneration by 2-3 folds. The promoting effect of SDf-1 was reduced by half when adding the receptor inhibitor AMD3100. Conclusions SDF-1 has promoting effects on axonal regeneration of retinal ganglion cells in retinal explants and has dose-response. （Ophtholmol CHN, 2012, 21： 256-260）
出处
《眼科》
CAS
2012年第4期256-260,共5页
Ophthalmology in China
基金
国家自然科学基金(81100931)
广东省自然科学基金(10451503102006299)
广东省科技计划(2010B031600132)
汕头科技计划(汕府科2010-63号)
汕头大学-香港中文大学联合汕头国际眼科中心院内科研基金(09-017)
