摘要
目的建立高效液相色谱法测定非甾体抗炎镇痛化合物4-邻甲苯磺酰氧基苯并噁唑酮(MBB)原料药的含量及有关物质。方法采用Odyssil C18(150 mm×4.6 mm,5μm)色谱柱,以甲醇-水(65∶35)为流动相,流速为0.8 mL.min 1,检测波长为273 nm,柱温为25℃。结果供试品中主成分峰与各杂质峰分离良好,MBB的线性范围为1～150μg.mL 1,回归方程为A=3 959.8C 2 831(r=0.999 7),平均回收率为99.2%(RSD=1.1%,n=9),检测限为1 ng。结论本方法简便、准确、专属性好,可用于MBB原料药的含量测定及有关物质检查。
OBJECTIVE To establish an HPLC method for determining the content of non-steroidal anti-inflammatory compound 4-O-methyl-benzenesulfonyl-2（3H）-benzoxazolone（MBB） and its related substances.METHODS The chromatographic separation was performed on an Odyssil C18 column（150 mm×4.6 mm,5 μm）.The mobile phase was consisted of methanol-water（65∶35） at a flow rate of 0.8 mL.min 1.The detection wavelength was set at 273 nm and the column temperature was 25 ℃.RESULTS MBB was completely separated from impurities.The linear range was 1 150 μg.mL 1,and the regression equation was A=3 959.8C 283 1（r=0.999 7）.The average recovery was 99.2%（RSD=1.1%,n=9）.The limit of detection was 1 ng.CONCLUSION The analytical method was simple,accurate and selective for the analysis of crude drug MBB and its related substances.
出处
《中国现代应用药学》
CAS
CSCD
2012年第9期833-835,共3页
Chinese Journal of Modern Applied Pharmacy
基金
山西省科技攻关项目(20110321076-01)
