摘要
目的:建立相同实验条件下芦丁、黄芩苷的静态吸附动力学方程,阐释黄酮类化合物结构对大孔树脂静态吸附的影响。方法:室温条件下,将等摩尔浓度的芦丁和黄芩苷溶液分别在AB-8树脂上进行静态吸附,分别采集相同时间点的样品,利用HPLC测定浓度,计算不同时间段的静态吸附量,绘制出静态吸附曲线,采用回归方法建立芦丁、黄芩苷的静态吸附动力学方程。结果:芦丁静态吸附方程为Y=4.715×X2+0.013×X1×X2;黄芩苷静态吸附方程为Y=4.921×X10.006×X2。二者的吸附动力学方程类型不同,在静态吸附上存在明显差异,表明结构对大孔树脂静态吸附具有显著性影响。结论:研究化合物结构与大孔树脂吸附影响的内在关系,对优化大孔树脂纯化工艺、提高提取效率和选择性具有理论价值和指导意义。
Static adsorption dynamics equations of Rutin and Baicalin were established on the basis of same ex- periment conditions to reveal and prove the effect of compound structure on static adsorption in maeroporous resin. At ambient temperature, the macroporous resin AB-8 was selected to adsorb Rutin and Baicalin in the same molarity conditions respectively. The adsorption data in the different time were determined by HPLC, and were counted according to the formulas. Then, static adsorption kinetic curves were drawn. And the static adsorp- tion dynamics equations of both Rutin and Baicalin were established by regression method. The static adsorption dynamics equation for Rutin is Y = 4.715×X2＋0.013×X1×X2, and for Baicalin is Y = 4.921×X0.0066×X2; where Y is static adsorption （mg/g）, X1 is the time of adsorbing （h）, and X2 is concentration of the sample （mg/ml）. The type of static adsorption dynamics equations and the static adsorptive rules of Rution and Baicalin are different. There are obvious differences. Results indicated that compounds structure of flavonoids have significant influence to macroporous resin static adsorption. It is very important to study the adsorptive internal relations of compound structures and macroporous resin. There are guiding roles and theoretical values to optimize the technology of ex- traction and to improve the extractive efficiency and selectivity. Keywords： Macroporous resin, flavonoids, compound structure, static adsorption, adsorption dynamics equation
出处
《世界科学技术-中医药现代化》
北大核心
2012年第4期1895-1898,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
北京中医药大学中青年教师资助项目(JYB22-JS031):基于分子构型的大孔树脂吸附动力学研究
负责人:马群
关键词
大孔树脂
黄酮类化合物
结构
静态吸附
吸附动力学方程
Macroporous resin,flavonoids,compound structure,static adsorption,adsorption dynamics equation
