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HMGB1促进外源DNA诱导细胞产生Ⅰ型干扰素 被引量：2

The enhancement of HMGB1 in exogenous DNA-induced type Ⅰ interferon production
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摘要 目的探讨HMGB1在外源DNA-poly(dA-dT)刺激细胞Ⅰ型干扰素IFN-β中的作用。方法荧光素酶分析方法检测经poly(dA-dT)刺激后细胞IFN-β的表达差异,同时PCR法检测了胞内重要DNA结合蛋白—高迁移率族蛋白-1(HMGB1)的表达改变;构建携带HMGB1特异性shRNA的慢病毒载体,获得HMGB1稳定下调(HMGB1KD)细胞,经poly(dA-dT)刺激后检测IFN-β表达,分析HMGB1在外源DNA刺激IFN-β产生中的作用。结果 poly(dA-dT)刺激293T细胞后,IFN-β表达显著上调,同时,HMGB1的表达在不同细胞中也明显上升,当下调HMGB1后,poly(dA-dT)刺激IFN-β产生的能力大幅削弱,表达水平降至对照组的1/8(P<0.05)。结论 HMGB1在促进外源DNA诱导Ⅰ型干扰素产生中发挥了关键作用。 The high mobility group box 1（HMGB1） protein is present in almost all eukaryotic cells and is highly conserved between species.Here,we explored the effect of HMGB1 on exogenous DNA-induced type Ⅰ interferon production in 293T cell.It was found that IFN-β was robustly induced in poly（dA-dT） stimulated cell;meanwhile,HMGB1 expression was also significantly increased in different cell lines.When knocking down cell HMGB1 expression by lentivirus-based shRNA strategy,IFN-β expression dramatically decreased to one eight of the original amount,indicating HMGB1 plays a key role in exogenous DNA-induced type Ⅰ interferon production.
作者 范兴娟 岳艳 董春升 胡静平 熊思东
机构地区 苏州大学生物医学研究院江苏省感染与免疫重点实验室
出处 《免疫学杂志》 CAS CSCD 北大核心 2012年第10期829-832,841,共5页 Immunological Journal
基金 教育部创新团队(IRT1075) 高校"青蓝工程"科技创新团队 江苏省"333高层次人才培养工程"培养对象第二层次专项基金
关键词 poly(dA-dT) Ⅰ型干扰素 HMGB1 Poly（dA-dT） Type Ⅰ interferon HMGB1
分类号 R392.12 [医药卫生—免疫学]
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参考文献25

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二级参考文献57

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共引文献22

同被引文献27

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免疫学杂志
2012年 第10期

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