摘要
通过COⅠ基因特异扩增测序,对徐闻地区的8种石珊瑚COⅠ基因片段序列进行了比较分析。结果表明,该片段的平均G+C含量为41.60%。其中,第1密码子位点的含量最高(42.26%～44.05%,平均为43.45%);转换和颠换多发生在第3密码子位点,比例高达70.30%和72.70%。采用临位连接(NJ)、最小进化(ME)和最大似然(ML)法对本研究中徐闻的8种及引自GenBank的20种石珊瑚COⅠ基因片段构建了系统发育树。结果显示,分子系统分类与传统分类略有差异,暗示珊瑚表型可塑性可能对传统分类存在影响。
Partial sequences of CO I genes from 8 species of Scleractinian in Xuwen area, Guangdong Province, were amplified and sequenced, and the base ratio and variations were ana- lyzed . The result indicated that the average G＋C content of this region accounted for 41.60%, while the eodon position 1 was the highest, in the range of 42.26%-44.05%（43.45% in average） . The proportion of transition and transversion was 70.30% and 72.70% respectively in codon position 3. The phylogenetic trees were also constructed based on CO I genes of these 8 samples and other 20 Scleractinian species recorded in GenBank. According to the Neighbour- joining, minimum-evolution and maximum-likelihood trees, molecular systematics was slightly different from the morphological conclusion, which suggested that morphological classification may be limited by coral skeletons plasticity. Consequently, the CO I gene is one of the auxiliary tools to classify the phylogeny of Scleractinia.
出处
《渔业科学进展》
CSCD
北大核心
2012年第4期83-88,共6页
Progress in Fishery Sciences
基金
国家海洋公益性行业科研专项(201105012)
广东省自然科学基金(S2011010000269)
广东省海洋渔业科技推广专项(A200908F01)共同资助
