摘要
利用ISSR分子标记技术对16个湖南柑橘主要品种进行了分子鉴定和亲缘关系分析。结果表明:9个引物共扩增出72条谱带,其中54条为多态性条带,多态率高达75%,平均每条引物扩增的DNA条带数目为8条。利用Popgene 1.32程序进行了Nei遗传距离和遗传相似性分析,橙类中彭娜和纽荷尔的亲缘关系最近。UPGMA聚类分析结果表明,在遗传距离为0.76的水平上,16个柑橘品种可分为四大类群。
Sixteen main varieties of citrus were used as materials for analyzing their genome polymorphism by ISSR markers. The results showed that total 72 bands were generated by nine primers, of which 54 bands were polymorphic bands （the percentage of polymorphic band, PPB=75%）. The average number of DNA fragments produced by each primer was eight. Nei similarity coefficients and genetic distances were calculated by using Popgene 1.32 software, and results indicated that Penna and Newhall have the closest relationship among citrus; the dendrogram were constructed by using UPGMA, and the results indicated that 16 citrus materials were divided into four major groups while the genetic distance was 0.76.
出处
《湖南农业科学》
2012年第9期117-119,122,共4页
Hunan Agricultural Sciences
基金
公益性行业(农业)科研专项(200903043-04)
湖南省农业科学院创新项目资助
关键词
湖南
柑橘
ISSR
聚类分析
遗传关系
Hunan
citrus
ISSR
cluster analysis
genetic relationship
