摘要
以掺杂了石墨烯纳米片的壳聚糖为功能基体,L-色氨酸为模板分子,利用恒电位沉积法制备对L-色氨酸具有手性识别功能的分子印迹传感器.采用扫描电子显微镜(SEM)、X射线衍射(XRD)、傅里叶变换红外光谱(FTIR)等方法表征印迹膜的形成过程.探讨印迹传感器的电化学性能,并优化了最优检测条件.研究结果表明:石墨烯掺杂量为1mg.mL-1,沉积时间为300s,工作电压为+0.85V,溶液pH值为6时,所制备的石墨烯-壳聚糖印迹传感器具有良好的手性识别性能,且对L-色氨酸的浓度线性响应范围为0.17～25μmol.L-1,检测限(S/N=3)达0.04μmol.L-1.
A novel electrochemical sensor was prepared by graphene-chitosan molecularly imprinted film,which casted on the gassy carbon electrode by electrodeposition,using L-tryptophan（L-Trp） as the template.The prepared film was investigated by scanning electron microscopy（SEM）,fourier transform infrared spectroscopy（FTIR） and X-ray diffraction（XRD）.The enantioselective performance and the optimal conditions for L-Trp detection were evaluated by electrochemical technique in detail.Under the optimum conditions of 1 mg·mL-1 graphene doping concentration,300 s electrodepositing time,the prepared sensor showed a excellent chiral recognition to Trp in pH=6 NaCl solution and linearly selective detection of L-Trp in the range of 0.17~25 μmol·L-1 was found,with a limit of detection of 0.04 μmol·L-1（S/N=3）.
出处
《华侨大学学报（自然科学版）》
CAS
北大核心
2012年第5期528-534,共7页
Journal of Huaqiao University(Natural Science)
基金
国家自然科学基金资助项目(20955001
21175049)
福建省自然科学基金计划资助项目(2011J01049)
中央高校基本科研业务费专项基金资助项目(10QZR13)
关键词
石墨烯
分子印迹传感器
手性识别
色氨酸
graphene
molecularly imprinted sensors
chiral recognitiony
tryptophan
