摘要
选用来自中国不同稻作生态的4个特殊常规水稻品种:辐恢838(普通籼稻)、月亮谷(云南高海拔籼稻)、C418(普通粳稻)和丽粳11号(云南高寒粳稻),于减数分裂期和开花期分别低温(16℃)处理5d后又恢复5d,研究低温下生殖生长期的冷胁迫效应(花粉育性,结实率,百粒重下降程度)及相关MADS-box基因差异表达情况。结果表明:四个品种生殖生长期耐冷性由低到高排列依次是:辐恢838<C418<月亮谷<丽粳11号。冷胁迫后5个MADS-box基因(OsMADS2,OsMADS3,OsMADS18,OsMADS26和OsMADS58)出现表达差异,其上调或下调程度因品种不同而异,OsMADS2在4个品种中都呈下调趋势,其余4个呈上调趋势,而OsMADS3、OsMADS58转录水平变化相似。此外,冷胁迫前后MADS-box基因在辐恢838和C418中差异表达程度较大,而在月亮谷和丽粳11号中差异表达程度小。结合基因差异表达情况与花粉育性、结实率等的评价结果,暗示这些MADS-box基因可能参与植物的冷胁迫反应,其上调或下调表达能够增强植物的抗冷性。该研究结果为解析水稻品种冷胁迫驯化及耐冷分子机制提供了新思路。
Four special conventional rice varieties from different ecological zones in China, namely Fuhui838 （common indica）, Yuelianggu （conventional indica landrace growing at high altitude in Yunnan）, C418 （common japonica） and Lijingl 1 （conventional japonica landrace growing in high altitude with cold climate in Yunnan）, were used to study the cold tolerance at reproductive stage under low temperature stress. At reproductive stage （meiosis and flowering）, the plants were treated at 16℃ for 5 days and then they were set to recover for 5 days under control. The pollen fertility, seed-setting rate and 100-grain weight were then measured, and the differential expression of MADS-box genes in rice was studied by RT-PCR. Results showed： the cold tolerance ranking from lower to higher were： Fuhui838 〈C418 〈Yuelianggu 〈Lijingl 1. Under low temperature stress, five MADS-box genes, OsMADS2, OsMADS3, OsMADS18, OsMADS26, and OsMADS58 expressed differentially and they were up or down regulated depending on the varieties. For example, OsMA DS2 was down-regulated in all four varieties, whereas the other four genes were up-regulated. Interestingly, OsMADS3 and OsMADS58 shared similar expression patterns. Moreover, it was found that the up- or down-regulated expression levels of MADS-box genes varied more in Fuhui838 and C418 than that in Yuelianggu and Lijingl 1. Piecing together observations of agronomic traits （pollen fertility, seed setting rate etc.） and differential expression of MADS-box genes under cold stress, the study results implied that the five MADS-box genes may participate in cold stress response in plants, and their up-or down-regulated expression could improve cold resistance in plants. Overall results of the present study provide a new insight into understanding of cold acclimation and molecular mechanisms of cold tolerance in rice varieties.
出处
《分子植物育种》
CAS
CSCD
北大核心
2012年第5期501-511,共11页
Molecular Plant Breeding
基金
NSFC-云南联合基金重点项目(U1136604)
国家重点基础研究973项目(2011CB100401)
云南省水稻产业技术体系(A3006516)共同资助
关键词
水稻
籼粳
耐冷性
冷害
MADS—box基因
Rice （Oryza sativa L.）, Irtdica and Japonica, Cold tolerance, Low temperature stress, MADS-box gene
