期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

自噬在血管紧张素Ⅱ诱导心肌细胞肥大中的作用 被引量：2

Role of Autophagy in Angiotensin Ⅱ Induced Cardiac Hypertrophy
下载PDF
导出
摘要 【目的】探讨自噬在血管紧张素(Ang)Ⅱ诱导心肌细胞肥大中的作用。【方法】原代培养的乳鼠心肌细胞,采用AngⅡ干预建立心肌肥大的模型;在光学显微镜下观察心肌细胞的形态,采用Real time PCR技术检测心肌细胞肥大标志物心房利钠多肽(ANP)的表达、Western blot法检测LC3蛋白的表达。【结果】AngⅡ可抑制自噬相关基因LC3蛋白的表达。自噬阻断剂3-甲基腺嘌呤(3MA)会促进AngⅡ诱导的心肌细胞肥大。【结论】AngⅡ可能通过抑制自噬来诱导心肌细胞肥大。 [ Objective ] To investigate the role of autophagy in cardiac hypertrophy induced by angiotensin Ⅱ （Ang Ⅱ ）. [ Methods ] Cardiac hypertrophy was induced by Ang Ⅱ incuhured neonatal rat cardiomyocytes. Morphology and expression of hypertrophy marker atrial natriuretic peptide （ANP） were detected by optical microscope and real time-PCR. Autophagic marker, LC3 level was tested by Western blotting. [ Results ] AngⅡ increased volume of cardiomyocyte, and up regulated RNA expression of ANP. Ang Ⅱ inhibited autophagy related gene LC3 protein expression. Blocking autophagy by 3-MA could significantly enhance myocardial hypertrophy induced by Ang Ⅱ. [ Conclusion] Ang Ⅱ may induce myocardial hypertrophy by inhibiting autophagy.
作者 汪海宁 刘晨 李燕辉 陈琮 孙刚 薛睿聪 董吁钢
机构地区 中山大学附属第一医院卫生部辅助循环重点实验室
出处 《中山大学学报（医学科学版）》 CAS CSCD 北大核心 2012年第4期440-443,448,共5页 Journal of Sun Yat-Sen University:Medical Sciences
基金 国家自然科学基金(81170226/H0212)
关键词 心肌细胞 心肌肥大 血管紧张素Ⅱ 自噬 相同基因 微管蛋白1轻链3 cardiomyocyte hypertrophy angiotensin Ⅱ （Ang Ⅱ ） autophagy related gene LC3 （ Tiny tubes related proteins 1light chain 3）
分类号 R363 [医药卫生—病理学]
  • 相关文献

参考文献15

  • 1Sugden PH, Clerk A. Cellular mechanisms of cardiac hypertrophy[J]. J Mol Med, 1998, 76 (11 ) : 725-746.
  • 2Shintani T, Klionky DJ. Autophagy in health and disease: a double-edged sword [J]. Science, 2004, 306 : 990-995.
  • 3陈广琴,刘晨,张成喜,蒙荣森,陈保林,熊肇军,董吁钢.HO-1在AMPK抑制心肌肥大中的作用[J].中山大学学报（医学科学版）,2010,31(5):614-618. 被引量：4
  • 4殷然,董吁钢,李红良,刘丹.AMPK通过增强心肌脂肪酸氧化抑制大鼠心肌肥厚[J].中国病理生理杂志,2007,23(7):1258-1262. 被引量：8
  • 5Huang WP, Klionsky DJ. Autophagy in yeast: a review of the molecular machinery [J]. Cell Struct Funct, 2002, 27 (6) : 409-420.
  • 6Levine B, Klionsky DJ. Development by self-digestion: molecular mechanisms and biological functions ofautophagy[J]. Dev Cell, 2004, 6(4) : 463-477.
  • 7Decker RS, Wildenthal K. Lysosomal alterations in hypoxic and reoxygenated hearts : I : Uhrastructural and cytochemical changes [ J ]. Am J Pathol, 1980, 98 (2) : 425 -444.
  • 8Dammrich J, Pfeifer U. Cardiac hypertrophy in rats after supravalvular aortic constriction : II : Inhibition of cellular autophagy in hypertrophying cardiomyocytes [J]. Virchows Arch B Cell Pathol Incl Mol Pathol, 1983, 43 (3) : 287-307.
  • 9Pfeifer U, Fohr J, Wilhelm W, et al. Short-term inhibition of cardiac cellular autophagy by isoproterenol [J]. J Mol Cell Cardiol, 1987, 19(12) : 1179 - 1184.
  • 10Nakai A, Yamaguchi O, Takeda T, et al. The role of autophagy in cardiomyocytes in the basal state and in response to hemodynamic stress [J]. Nat Med, 2007, 13: 619-624.

二级参考文献30

  • 1Kemp BE,Mitchelhill KI,Stapleton D,et al.Dealing with energy demand:the AMP-activated protein kinase[J].Trends Biochem Sci,1999,24(1):22-25.
  • 2Carling D.AMP-activated protein kinase:balancing the scales[J].Biochimie,2005,87(1):87-91.
  • 3Stapleton D,Mitchelhill KI,Gao G,et al.Mammalian AMP-activated protein kinase subfamily[J].J Biol Chem,1996,271(2):611-614.
  • 4baron SJ,Li J,Russell RR,et al.Dual mechanisms regulating AMPK kinase action in the ischemic heart[J].Circ Res,2005,96(3):337-345.
  • 5Saberi B,Shinohara M,Ybanez MD,et al.Regulation of H2O2-induced necrosis by PKC and AMP-activated kinase signaling in primary cultured hepatocytes[J].Am J Physiol Cell Physiol,2008,295(1):C50-63.
  • 6MS G.AMP-activated protein kinase is activated as a consequence of lipolysis in the adipocyte:potential mechanism and physiological relevance[J].J Biol Chem,2008,283(24):16514-16524.
  • 7Chan AY,Soltys CL,Young ME,et al.Activation of AMP-activated protein kinase inhibits protein synthesis associated with hypertrophy in the cardiac myocyte[J].J Biol Chcm,2004,279(31):32771-32779.
  • 8Foresti R,Goady H,Green C J,et al.Role of heme oxygenase-1 in hypoxia-reoxygenation:requirement of substrate heme to promote cardioprotection[J].Am J Physiol Heart Circ Physiol,2001,281(5):H1976-1984.
  • 9Hayashi S,Takamiya R,Yamaguchi T,et al.Induction of heme oxygenase-1 suppresses venular leukocyte adhesion elicited by oxidative stress:role of bilirubin generated by the enzyme[J].Circ Res,1999,85(8):663-671.
  • 10Katori M,Buelow R,Ke B,et al.Heme oxygenase-1overexpression protects rat hearts from cold ischemia/reperfusion injury via an antiapoptotic pathway[J].Transplantation,2002,73(2):287-292.

共引文献10

同被引文献19

  • 1Conn PF,Schalch W,Truscott TG. The singlet oxygen and carotenoid interaction [J]. J Photochem Photobiol B, 1991,11 ( 1 ) :41-47.
  • 2Miller N J, Sampson J, Candeias LP, et al. Antioxidant activities of carotenes and xanthophylls [J]. FEBS Lett, 1996,384(3) :240- 242.
  • 3Simone RE, Russo M, Catalano A, et al. Lycopene inhibits NF- kB-mediated IL-8 expression and changes redox and PPAR gam- ma signalling in cigarette smoke-stimulated macrophages [J]. PLoS ONE, 2011,6 (5) : e 19652.
  • 4Kang M,Park KS,Seo JY,et al. Lycopene inhibits IL-6 ex- pression in cerulein-stimulated pancreatic acinar cells [J]. Genes Nutr,2011,6(2) : 117-123.
  • 5Shukla SK,Gupta S,Ojha SK,et al. Cardiovascular friendly natural products:a promising approach in the management of CVD [J]. Nat Prod Res,2010,24(9):873-898.
  • 6Lo HM,Hung CF,Tseng YL,et al. Lycopene binds PDGF-BB and inhibits PDGF-BB-induced intracellular signaling transduc- tion pathway in rat smooth muscle cells [J]. Biochem Pharma- col, 2007,74 ( 1 ) : 54-63.
  • 7Shintani T,Klionky DJ. Autophagy in health and disease:a double-edged sword [J]. Science ,2004,306(5698):990-995.
  • 8Huang WP,Klionsky DJ. Autophagy in yeast:a review of the molecular machinery [J]. Cell Struct Funct,2002,27(6) :409-420.
  • 9Giovannucci E,Ascherio A,Rimm EB,et al. Intake ofcarotenoids and retinol in relation to risk of prostate cancer [J]. J Natl Can- cer Inst, 1995,87(23) : 1767-1776.
  • 10Hu MY, Li YL,Jiang. CH,et ah Comparison of lycopene and fluvastatin effects on atherosclerosis induced by a high-fat diet in rabbits [J]. Nutrition ,2008,24(10) : 1030-1038.

引证文献2

二级引证文献10

中山大学学报（医学科学版）
2012年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部