摘要
[目的]观察乌梅丸对溃疡性结肠炎大鼠结肠组织白细胞介素(IL)-8、IL-13的影响。[方法]84只SD大鼠随机分为正常对照组,模型组,柳氮磺胺吡啶(SASP)组,乌梅丸大、中、小剂量组,每组14只;除正常组外,其他各组大鼠采用2,4-二硝基氯苯(DNCB)加醋酸复合法制备溃疡性结肠炎模型;采用双抗体夹心ELISA法检测各组大鼠结肠组织IL-8、IL-13的含量。[结果]模型组大鼠结肠组织中IL-8含量较正常组显著增高(P<0.01),乌梅丸各剂量组、SASP组则较模型组明显降低(P<0.01或P<0.05);模型组大鼠结肠组织IL-13含量较正常组显著降低(P<0.01),乌梅丸各剂量组、SASP组较模型组明显升高(P<0.01或P<0.05)。乌梅丸大剂量组降低IL-8、升高IL-13的效果最显著,其IL-8、IL-13的含量与正常组比较差异无统计学意义。[结论]IL-8、IL-13参与了UC的发生、发展,乌梅丸能通过降低溃疡性结肠炎大鼠结肠组织IL-8的含量、升高IL-13的含量,从而起到治疗UC的作用。
[Objective] To detect the effect of Wumei Pill treatment on the expression of interleukin-8（IL-8） and interleukin-13（IL-13） in the colon tissue of the rats with ulcerative colitis.[Methods]Eighty-four SD rats were randomly divided into six groups,namely the normal control group,the model group,the solfasalazine（SASP）group,the Wumei Pill high dosage group,medium dosage group,low-dosage group.Except the normal control group,the other five groups of UC model was made through the integration of general immunity and local stimulation of DNCB and acetic acid.Thereafter,the method of immunohistochemistry was used to detect the content of IL-8 and IL-13 in the colon tissue.[Results]Compared with normal control group,the content of IL-8 increased and that of IL-13 decreased significantly in all model rats（P〈0.01）.Compared with that or the model group,the content of IL-8 in Wumei Pill groups and SASP group reduced markedly（P〈0.01） and the content of IL-13 increased markedly（P〈0.01）.The change of IL-8 and IL-13 contents was most remarkable in the Wumei Pill high-dosage group.[Conclusion]IL-8 and IL-13 were related to the occurrence and development of UC.Wumei Pill plays a role in the treatment of UC,which might be partly due to the chage of IL-8 and IL-13 contents.
出处
《中国中西医结合消化杂志》
CAS
2012年第9期409-411,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
