摘要
通过对圆弧青霉PG3 7所产碱性脂肪酶活性测定方法的研究和比较 ,建立了一种快速、直观、灵敏的固体琼脂平板脂肪酶活性测定方法 .利用脂肪酶在一定条件下分解三丁酸甘油酯所释放出的游离脂肪酸使指示剂 (维多利亚蓝 )变色的原理 ,根据变色圈直径的大小对酶活性进行粗略分析 ,特别适合于酶提纯过程中大批量样品酶活性的估测以及酶活性条带和其它蛋白质条带的区分 .
Based on studies and comparison among the assay of the activity of alkaline lipase produced by Penicillium cyclopium PG37, a rapid, direct and sensitive solid agar plate method for the assay of the activity of alkaline lipase was developed. The underlying principle employed in this method was based on colour change of the indicator(Victoria Blue) caused by free fatty acids liberated from tributyrin under proper conditions. Lipase activity was analyzed quickly and riughly according to the diameters of the halos. The method specially fitted for the assay of the activity of lipases in large series of samples collected in enzyme purification and the differentiation of activity bands of proteins and other bands of proteins.
基金
国家"九五"科技攻关项目资助!课题 ( 96-C0 3- 0 2 - 0 1 )
