摘要
目的 :探索蝎毒抗癌多肽组分Ⅲ (antineoplasticpolypeptide ⅢfromAPBMV ,AP Ⅲ )的分离和纯化方法。方法 :蝎毒经预处理后 ,分别采用SepharoseFF阳离子交换凝胶柱 (2 5cm× 5cm交换柱 ,流速 0 .8ml/min ,梯度洗脱1 2 0 0min)层析法和SepharoseFF阳离子交换凝胶柱与SephadexG 5 0凝胶柱 (柱 :1 0 0cm× 5cm ;流速 :0 .5ml/min)联合层析法 ,分离AP Ⅲ ,用HPLC仪检测 2种方法分离出的AP Ⅲ的纯度及产率。结果 :单独用SepharoseFF阳离子交换凝胶柱分离 ,AP Ⅲ产率为 2 .2 4% ,纯度为 89%。SephadexG 5 0凝胶柱与SepharoseFF阳离子交换凝胶柱联用 ,其产率及纯度分别提高至 4.72 %和 95 %。结论 :SephadexG 5 0与SepharoseFF联用可提高AP Ⅲ的产率及纯度 ,从而为开发和利用AP
Aim: To explore the methods on isolation and purification of antineoplastic polypeptide Ⅲ from APBMV (AP Ⅲ). Methods: The AP Ⅲ was isolated from scorpion venom of Buthus martensii karsch by pretreatment, ion exchange chromatography (Sepharose FF, column:25 cm×5 cm,flow rate:0.8 ml/min,linear gradient:1 200 min) or coupling gel filtration(Sephadex G 50, column:100 cm×5 cm,flow rate:0.5 ml/min) with Sepharose FF chromatography. The purity of AP Ⅲ and other compositions of scorpion venom were inspected by high performance liquid chromatography. Results:The purification rate and output of AP Ⅲ were 89% and 2.24% with ion exchange chromatography only,but 95% and 4.72% with coupling Sephadex G 50 gel filtration with Sepharose FF chromatography.Conclusion:The fractionating method of coupling Sephadex G 50 gel filtration with Sepharose FF chromatography is useful for improving the purification rate and output of AP Ⅲ. The experimental result is thought as an important evidence to make clinical use of AP Ⅲ in treatment of tumor.
出处
《河南医科大学学报》
2000年第4期282-285,共4页
Journal of Henan Medical University
基金
河南省"九五"科技攻关项目! 95 12 0 0 10 2
