摘要
目的:观察不同剂量低分子肝素钠对急性DVT大鼠在不同时期治疗后血管形态学的变化。方法:将72只SD大鼠制备模型后,随机按血栓形成后1、3、7、14d分期,每期18只,分为Ⅰ期、Ⅱ期、Ⅲ期、Ⅳ期。然后每期再根据低分子肝素钠剂量分为:低分子肝素钠小剂量组(700U/kg、一次/24h,n=6)、低分子肝素钠大剂量组(700U/kg、二次/24h,n=6)、模型组(0U/kg,n=6)。低分子肝素钠大、小剂量组均由大鼠腹壁皮下注射,连续7日。实验结束后获取病变段血管,观察内皮细胞数量变化,胶原阳性染色面积百分比变化,并与同期模型组进行比较。结果:模型组在Ⅱ期、Ⅲ期分别死亡一只,低分子肝素钠治疗大剂量组Ⅰ期、Ⅲ期、Ⅳ期内死亡4只。在Ⅰ期低分子肝素钠大、小剂量组内皮细胞数量与模型组相比均有统计学差异(P<0.05),组间亦有差异.低分子肝素钠大、小剂量组胶原阳性染色面积百分比与模型组相比均有统计学差异(P<0.05),组间相比无差异;在Ⅱ期,低分子肝素钠大、小剂量组内皮细胞数量与模型组相比均有统计学差异(P<0.05),组间无差异;在Ⅲ期、Ⅳ期低分子肝素钠大、小剂量组内皮细胞数量与模型组相比无统计学差异(P>0.05);在Ⅱ期、Ⅲ期低分子肝素钠大、小剂量组胶原阳性染色面积百分比与模型组相比仍有统计学差异(P<0.05),组间亦有差异;在Ⅳ期低分子肝素钠大、小剂量组胶原阳性染色面积百分比与模型组相比无统计学差异(P>0.05)。结论:血栓形成3d内,大、小剂量低分子肝素钠对内皮细胞均有保护作用,以大剂量效果较好。血栓形成7d内,大、小剂量低分子肝素钠对对胶原增生均有抑制作用,随着时间的延长,以大剂量低分子肝素钠抑制作用最强。
Objective： To observe the effect of different dose, s Low molecular weight heparin（LMWH） on morphological changes of vein wall after differenct periods of deep vein thrombosis （DVT） in rats. Methods： Firstly,72 sprague- dawley （SD） rats were randomly divided into Ⅰ , Ⅱ, Ⅲ,Ⅳ periods on lst,3rd, 7th, 14th day following thrombosis respectively, there were 18 SD rats in every period. Then the 18 SD rats in each period were separated to 4 group. Low dose＇s Low molecular weight heparin（LMWH） group （700u/kg, 1t/24h, n =6. ） ,high dose＇s Low molecular weight heparin （LMWH） group（700u/kg, 1t/12h, n = 6. ）, thrombosis group（0,000u/kg, n =6）. In each group, to observe the remain quantity of endothelial cells and the expression areas of collagen by dyeing. Results： 2 SD rats in thrombosis group were died in Ⅱ, Ⅲ period respectively. 4 SD rats in high doseg Low molecular weight heparin（LMWH） group were died in Ⅰ ,Ⅲ,Ⅳ period in thrombolytic therapy. In Ⅰ period, the quantity of endothelial cells in low,high doseg LMWH group have significant difference with thrombosis group respectively （ P 〈 0.05）. The difference exisited in interclass as well（ P 〈 0.05 ）. In I period, the expression areas of collagen in low,high dose＇s LMWH group have significant difference with thrombosis group respectively （P 〈 0.05 ） ; In Ⅱ period, the quantity of endothelial cells in low,high dose＇s LMWH group have significant difference with thrombosis group respectively （ P 〈 0.05 ） , The difference do not exisited in interelass （ P 〉 0.05） ; In Ⅲ, Ⅳ periods, the quantity of endothelial cells in low ,high dose＇s LMWH group haven, t significant difference with thrombosis group respectively（ P 〉 0.05 ）. In Ⅱ, Ⅲ period, the expression areas of collagen in low,high dose＇s LMWH group have significant difference with thrombosis group respectively （P 〈0.05 ）, The difference exisited in interclass as well（ P 〈 0.05 ）. In Ⅳ period, The expression areas of collagen in low,high dose＇s LMWH group haven ,t significant difference with thrombosis group respectively （ P 〈 0.05 ） . Conclusions： Different dose, s LMWH have protective effect on the quantity of endothelial cells during 3 days after thrombosis, the effect of highdose＇s LMWH is better; Different dose, s LMWH haven, t protective on the quantity of endothelial cells after 7 days following thrombosis, but they have inhibitary effect on the expression areas of collagen in every period, the effect of high dose＇s LMWH is better.
出处
《新疆医学》
2012年第9期35-40,共6页
Xinjiang Medical Journal
关键词
低分子肝素钠
深静脉血栓形成
形态学变化
Low molecular weight heparin（LMWH）
deep venous thrombosis （DVT）
morphologicalchanges
