摘要
利用微波-超声波辅助方法合成了水溶性聚乙烯亚胺包覆Mn掺杂ZnS量子点,并建立了基于该量子点的室温磷光检测三磷酸鸟苷(GTP)的新方法。该量子点对GTP具有较高的灵敏度和选择性,能区分GTP与其结构类似物三磷酸腺苷(ATP)、三磷酸胞苷(CTP)、三磷酸尿苷(UTP)、二磷酸腺苷(ADP)和一磷酸腺苷(AMP)。该量子点的室温磷光对GTP的响应在3 min内达到平衡,其磷光增强值与GTP浓度呈良好的线性关系,对GTP的检出限为0.6μmol/L。该方法成功应用于癌细胞提取液中GTP的检测,为GTP的检测提供了新方法。
High quality PEI-capped Mn-doped ZnS QDs were prepared via a microwave and ultrasonic-assisted synthesis for room temperature phosphorescence detection of guanosine 5＇-triphosphate（GTP）.Such QDs showed high selectivity and sensitivity for GTP,and could distinguish GTP from adenosine triphosphate（ATP）,cytidine triphosphate（CTP）,uridine triphosphate（UTP）,adenosine diphosphate（ADP） and adenosine monophosphate（AMP）.The room temperature phosphorescence response of the PEI-capped Mn-doped ZnS QDs for GTP reached equilibrium in 3 min.PEI-capped Mn-doped ZnS QDs gave a detection limit（3s） of 0.6 μmol/L for GTP.The developed phosphorescence probe favors biological applications since the interference from scattering light and autofluorescence can be effectively eliminated.The developed method has been successfully applied for the room temperature phosphorescence detection of GTP in cancer cell extract.
出处
《分析测试学报》
CAS
CSCD
北大核心
2012年第9期1042-1050,共9页
Journal of Instrumental Analysis
基金
国家自然科学基金(20935001)
天津市自然科学基金(10JCZDJC16300)
关键词
Mn掺杂ZnS量子点
微波超声波辅助合成
室温磷光
三磷酸鸟苷
Mn-doped ZnS quantum dots
microwave and ultrasonic-assisted synthesis
room temperature phosphorescence
guanosine 5＇-triphosphate
