摘要
研究了一种基于单光子计数技术的新型半自动化学发光免疫分析仪。利用光电倍增管(PMT,Photomultiplier)作为微弱荧光的检测元件,采用单光子计数技术来测量微弱荧光光强,并且使用自主设计的测量暗室和聚光光路来提高光子采集效率。以浓度为3mIU/ml的卵泡刺激素(FSH)为例,分别用不加聚光光路、单独加抛物面反射器、加了聚光光路测量其反应后的光子数值,所得光子数平均值分别是91 610.8、115 307.4、149 500.8,单独加抛物面反射器和加上聚光光路后的光子采集效率比不加聚光光路时分别提高了25.86%和63.19%。设计的新型半自动化学发光免疫分析仪采用自主研发的测量暗室和聚光光路,极大的提高了测量结果的准确性。
A new semi-automatic chemiluminescence immunoassay analyzer was developed with the technology of photons counting.The analyzer used PMT as the detector of the weak fluorescence with photons counting,and used the measuring chamber and the optical system to improve the fluorescence collection.The number of FSH（3mIU/ml）’s photons is on an average of 1 610.8,115 307.4 and 149 500.8 respectively,which were tested by the photons counter without the optical system,with the parabolic reflector and with the optical system in turn.Obviously,the last two photons counters raised the photonic collection efficiency by 25.86% and 63.19% respectively.The veracity of the analyzer was increased by using the measuring chamber and the optical system.
出处
《光电子技术》
CAS
北大核心
2012年第3期190-194,共5页
Optoelectronic Technology
基金
吉林省科技发展计划项目(编号:20070327)
关键词
化学发光免疫分析
光电倍增管
测量暗室
聚光光路
chemiluminescence immunoassay； PMT； measuring chamber； rays convergence optical system
